Number of the records: 1
The translational oscillation in oocyte and early embryo development
- 1.
SYSNO ASEP 0580751 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The translational oscillation in oocyte and early embryo development Author(s) Iyyappan, Rajan (UZFG-Y) ORCID
Aleshkina, Daria (UZFG-Y)
Ming, H. (US)
Dvořan, Michal (UZFG-Y) ORCID
Kakavand, Kianoush (UZFG-Y)
Jansová, Denisa (UZFG-Y) ORCID
del Llano, Edgar (UZFG-Y) ORCID
Gahurová, Lenka (UZFG-Y) ORCID
Bruce, A. W. (CZ)
Mašek, T. (CZ)
Pospíšek, M. (CZ)
Horvat, Filip (UMG-J)
Kubelka, Michal (UZFG-Y) RID, ORCID
Jiang, Z. (US)
Šušor, Andrej (UZFG-Y) RID, ORCIDSource Title Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 51, Nov 23 (2023), s. 12076-12091Number of pages 16 s. Publication form Print - P Language eng - English Country US - United States Keywords translation ; oocyte ; early embryo Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology R&D Projects GA22-27301S GA ČR - Czech Science Foundation (CSF) EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 ; UMG-J - RVO:68378050 UT WOS 001100405100001 EID SCOPUS 85180007080 DOI 10.1093/nar/gkad996 Annotation Translation is critical for development as transcription in the oocyte and early embryo is silenced. To illustrate the translational changes during meiosis and consecutive two mitoses of the oocyte and early embryo, we performed a genome-wide translatome analysis. Acquired data showed significant and uniform activation of key translational initiation and elongation axes specific to M-phases. Although global protein synthesis decreases in M-phases, translation initiation and elongation activity increases in a uniformly fluctuating manner, leading to qualitative changes in translation regulation via the mTOR1/4F/eEF2 axis. Overall, we have uncovered a highly dynamic and oscillatory pattern of translational reprogramming that contributes to the translational regulation of specific mRNAs with different modes of polysomal occupancy/translation that are important for oocyte and embryo developmental competence. Our results provide new insights into the regulation of gene expression during oocyte meiosis as well as the first two embryonic mitoses and show how temporal translation can be optimized. This study is the first step towards a comprehensive analysis of the molecular mechanisms that not only control translation during early development, but also regulate translation-related networks employed in the oocyte-to-embryo transition and embryonic genome activation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2024 Electronic address https://academic.oup.com/nar/article/51/22/12076/7405371?login=true
Number of the records: 1