Continuous short-term acclimation to moderate cold elicits cardioprotection in rats, and alters β-adrenergic signaling and immune status

Marvanová, A.; Kašík, P.; Elsnicová, B.; Tibenská, V.; Galatík, F.; Horníková, D.; Zvolská, V.; Vebr, P.; Vodička, Petr; Hejnová, L.; Matouš, P.; Szeiffová Bačová, B.; Sýkora, M.; Novotný, J.; Neužil, Jiří; Kolář, František; Nováková, Olga; Žurmanová, J.

doi:https://dx.doi.org/10.1038/s41598-023-44205-4

Number of the records: 1

Continuous short-term acclimation to moderate cold elicits cardioprotection in rats, and alters β-adrenergic signaling and immune status

1.

SYSNO ASEP	0580333
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Continuous short-term acclimation to moderate cold elicits cardioprotection in rats, and alters β-adrenergic signaling and immune status
Author(s)	Marvanová, A. (CZ) Kašík, P. (CZ) Elsnicová, B. (CZ) Tibenská, V. (CZ) Galatík, F. (CZ) Horníková, D. (CZ) Zvolská, V. (CZ) Vebr, P. (CZ) Vodička, Petr (UZFG-Y)_ORCID Hejnová, L. (CZ) Matouš, P. (CZ) Szeiffová Bačová, B. (SK) Sýkora, M. (CZ) Novotný, J. (CZ) Neužil, Jiří (BTO-N)_RID Kolář, František (FGU-C)_{RID, ORCID, SAI} Nováková, Olga (FGU-C) Žurmanová, J. (CZ)
Article number	18287
Source Title	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 13, č. 1 (2023)
Number of pages	18 s.
Publication form	Online - E
Language	eng - English
Country	US - United States
Keywords	moderate cold acclimation ; cardioprotection ; rats
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Biochemistry and molecular biology
R&D Projects	LX22NPO5104 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
Method of publishing	Open access
Institutional support	UZFG-Y - RVO:67985904 ; BTO-N - RVO:86652036 ; FGU-C - RVO:67985823
UT WOS	001089332200020
EID SCOPUS	85174908332
DOI	10.1038/s41598-023-44205-4
Annotation	Moderate cold acclimation (MCA) is a non-invasive intervention mitigating effects of various pathological conditions including myocardial infarction. We aim to determine the shortest cardioprotective regimen of MCA and the response of beta 1/2/3-adrenoceptors (beta-AR), its downstream signaling, and inflammatory status, which play a role in cell-survival during myocardial infarction. Adult male Wistar rats were acclimated (9 degrees C, 1-3-10 days). Infarct size, echocardiography, western blotting, ELISA, mitochondrial respirometry, receptor binding assay, and quantitative immunofluorescence microscopy were carried out on left ventricular myocardium and brown adipose tissue (BAT). MultiPlex analysis of cytokines and chemokines in serum was accomplished. We found that short-term MCA reduced myocardial infarction, improved resistance of mitochondria to Ca2+-overload, and downregulated beta 1-ARs. The beta 2-ARs/protein kinase B/Akt were attenuated while beta 3-ARs translocated on the T-tubular system suggesting its activation. Protein kinase G (PKG) translocated to sarcoplasmic reticulum and phosphorylation of AMPKThr172 increased after 10 days. Principal component analysis revealed a significant shift in cytokine/chemokine serum levels on day 10 of acclimation, which corresponds to maturation of BAT. In conclusion, short-term MCA increases heart resilience to ischemia without any negative side effects such as hypertension or hypertrophy. Cold-elicited cardioprotection is accompanied by beta 1/2-AR desensitization, activation of the beta 3-AR/PKG/AMPK pathways, and an immunomodulatory effect.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2024
Electronic address	https://www.nature.com/articles/s41598-023-44205-4

Number of the records: 1