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Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis
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SYSNO ASEP 0579429 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesis Author(s) Vaníčková, Karolína (UMG-J)
Miloševič, Mirko (BTO-N)
Ribeiro Bas, Irina (UMG-J) ORCID
Burocziová, Monika (UMG-J)
Yokota, A. (JP)
Daněk, Petr (UMG-J)
Grušanovič, Srdjan (UMG-J)
Chilinski, M. (PL)
Plewczynski, D. (PL)
Rohlena, Jakub (BTO-N) RID, ORCID
Hirai, H. (JP)
Rohlenová, Kateřina (BTO-N) ORCID, RID
Alberich-Jorda, Meritxell (UMG-J) RIDNumber of authors 13 Article number e113527 Source Title EMBO Journal. - : Wiley - ISSN 0261-4189
Roč. 42, č. 23 (2023)Number of pages 18 s. Language eng - English Country US - United States Keywords protein-c receptor ; c/ebp-beta ; progenitor cells ; self-renewal ; steady-state ; gene ; differentiation ; signals ; driven ; cd201 ; cd201 ; emergency granulopoiesis ; lymphoid-biased HSC ; myeloid-biased HSC OECD category Cell biology R&D Projects LX22NPO5102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA22-18300S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UMG-J - RVO:68378050 ; BTO-N - RVO:86652036 UT WOS 001083429900001 EID SCOPUS 85174320525 DOI 10.15252/embj.2023113527 Annotation Emergency granulopoiesis is the enhanced and accelerated production of granulocytes that occurs during acute infection. The contribution of hematopoietic stem cells (HSCs) to this process was reported, however, how HSCs participate in emergency granulopoiesis remains elusive. Here, using a mouse model of emergency granulopoiesis we observe transcriptional changes in HSCs as early as 4 h after lipopolysaccharide (LPS) administration. We observe that the HSC identity is changed towards a myeloid-biased HSC and show that CD201 is enriched in lymphoid-biased HSCs. While CD201 expression under steady-state conditions reveals a lymphoid bias, under emergency granulopoiesis loss of CD201 marks the lymphoid-to-myeloid transcriptional switch. Mechanistically, we determine that lymphoid-biased CD201+ HSCs act as a first response during emergency granulopoiesis due to direct sensing of LPS by TLR4 and downstream activation of NF-kappa Beta signaling. The myeloid-biased CD201- HSC population responds indirectly during an acute infection by sensing G-CSF, increasing STAT3 phosphorylation, and upregulating LAP/LAP* C/EBP beta isoforms. In conclusion, HSC subpopulations support early phases of emergency granulopoiesis due to their transcriptional rewiring from a lymphoid-biased to myeloid-biased population and thus establishing alternative paths to supply elevated numbers of granulocytes. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2024 Electronic address https://www.embopress.org/doi/epdf/10.15252/embj.2023113527?src=getftr
Number of the records: 1