Epitachophoresis – New insert for horizontal gel electrophoresis device

0577806 - ÚIACH 2024 CZ eng A - Abstract
Vaňková, Markéta - Voráčová, Ivona - Hlaváček, Antonín - Foret, František
Epitachophoresis – New insert for horizontal gel electrophoresis device.
CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2023. s. 73-73. ISBN 978-80-908154-0-7.
[CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./. 23.10.2023-25.10.2023, Brno]
Institutional support: RVO:68081715
Keywords : epitachophoresis (ETP) * DNA fragments * DNA analysis * ETP device
OECD category: Analytical chemistry
https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf

This work focuses on the use of epitachophoresis (ETP) for the separation, purification, concentration, and isolation of DNA fragments. A new type of ETP device was developed and tested. The uniqueness of the new device is in its design and structure since it will be used as a removable extension for horizontal gel electrophoresis devices. Generally, the presented device can be used for DNA extraction, either in isotachophoretic or electrophoretic mode. Depending on the initial conditions, DNA concentration and/or separation is possible. Firstly, the functionality of the new ETP device was tested. The following steps were selecting a gel stabilization medium, optimizing DNA sampling, and determining DNA yield. The DNA fragments were visualized using SYBR Gold fluorescent stain.
Permanent Link: https://hdl.handle.net/11104/0346901