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Optimization of glycoproteomic protocol for efficient and sensitive analysis of N-Linked glycans by CE/LIF
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SYSNO ASEP 0577620 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Optimization of glycoproteomic protocol for efficient and sensitive analysis of N-Linked glycans by CE/LIF Author(s) Bobálová, Janette (UIACH-O) RID, ORCID
Smolková, Denisa (UIACH-O)
Strouhalová, Dana (UIACH-O)
Čmelík, Richard (UIACH-O) RID
Lavická, Jana (UIACH-O) ORCID, RIDNumber of authors 5 Source Title CECE 2023. 18th International Interdisciplinary Meeting on Bioanalysis. - Brno : Ústav analytické chemie AV ČR, v. v. i., 2023 - ISBN 978-80-908154-0-7
S. 44-44Number of pages 1 s. Publication form Online - E Action CECE 2023. International Interdisciplinary Meeting on Bioanalysis /18./ Event date 23.10.2023 - 25.10.2023 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords CE/LIF analysis ; cascade blue hydrazide ; glycans Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA22-00236S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation In this work, we report the effect of different proteomic protocols varying in enzyme or digestion procedures followed by derivatization by Cascade Blue hydrazide (8-(2-hydrazino-2-oxoethoxy)pyrene-1,3,6-trisulfonic acid trisodium salt, CBH) on the glycan detection in CE/LIF analysis. CBH represents a more reactive analog of APTS widely used for glycan labeling before CE analysis [1]. Ribonuclease B was selected as a model glycoprotein and four digestion protocols (alkylation, in gel/in solution treatment, purification step, etc.) were applied in order to obtain highly sensitive detection of labeled glycans by CE/LIF analysis. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2024 Electronic address https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf
Number of the records: 1