Number of the records: 1
A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley
- 1.
SYSNO ASEP 0577172 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley Author(s) Chen, C. (GB)
Jost, M. (AU)
Outram, M. A. (AU)
Friendship, D. (AU)
Chen, J. (GB)
Wang, A. (US)
Periyannan, S. (AU)
Bartoš, Jan (UEB-Q) RID, ORCID
Holušová, Kateřina (UEB-Q) ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Zhang, P. (AU)
Bhatt, D. (AU)
Singh, D. (AU)
Lagudah, E. (AU)
Park, R.F. (AU)
Dracatos, P. M. (AU)Number of authors 16 Article number 5468 Source Title Nature Communications. - : Nature Publishing Group - ISSN 2041-1723
Roč. 14, č. 1 (2023)Number of pages 11 s. Language eng - English Country US - United States Keywords PUCCINIA-HORDEI ; GENE RPH7 ; IDENTIFICATION OECD category Plant sciences, botany Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 001066569000014 EID SCOPUS 85169998926 DOI https://doi.org/10.1038/s41467-023-41021-2 Annotation Leaf rust, caused by Puccinia hordei, is one of the most widespread and damaging foliar diseases affecting barley. The barley leaf rust resistance locus Rph7 has been shown to have unusually high sequence and haplotype divergence. In this study, we isolate the Rph7 gene using a fine mapping and RNA-Seq approach that is confirmed by mutational analysis and transgenic complementation. Rph7 is a pathogen-induced, non-canonical resistance gene encoding a protein that is distinct from other known plant disease resistance proteins in the Triticeae. Structural analysis using an AlphaFold2 protein model suggests that Rph7 encodes a putative NAC transcription factor with a zinc-finger BED domain with structural similarity to the N-terminal DNA-binding domain of the NAC transcription factor (ANAC019) from Arabidopsis. A global gene expression analysis suggests Rph7 mediates the activation and strength of the basal defence response. The isolation of Rph7 highlights the diversification of resistance mechanisms available for engineering disease control in crops. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1038/s41467-023-41021-2
Number of the records: 1