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MUC13-miRNA-4647 axis in colorectal cancer: Prospects to identifications of risk factors and clinical outcomes
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SYSNO ASEP 0577152 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title MUC13-miRNA-4647 axis in colorectal cancer: Prospects to identifications of risk factors and clinical outcomes Author(s) Sojka, L. (CZ)
Opattová, Alena (UEM-P)
Bártů, Linda (UEM-P)
Horák, Josef (UEM-P) ORCID
Kořenková, V. (CZ)
Novosadová, Vendula (UMG-J)
Křížková, V. (CZ)
Brůha, Jan (UEM-P)
Liška, Václav (UEM-P) ORCID, RID
Schneiderová, M. (CZ)
Kubeček, O. (CZ)
Vodičková, Ludmila (UEM-P) RID
Urbanová, Markéta (UEM-P)
Šimša, J. (CZ)
Vodička, Pavel (UEM-P) RID
Vymetálková, Veronika (UEM-P) RIDArticle number 72 Source Title Oncology Letters. - : Spandidos Publications - ISSN 1792-1074
Roč. 25, č. 2 (2023)Number of pages 14 s. Language eng - English Country GR - Greece Keywords colorectal cancer risk and clinical outcomes ; MUC13 ; microRNA ; translation research OECD category Biochemistry and molecular biology R&D Projects LX22NPO5102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA22-05942S GA ČR - Czech Science Foundation (CSF) NV19-09-00237 GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support UEM-P - RVO:68378041 ; UMG-J - RVO:68378050 UT WOS 000915155300001 EID SCOPUS 85146013482 DOI 10.3892/ol.2022.13658 Annotation MUC13, a transmembrane mucin glycoprotein, is overexpressed in colorectal cancer (CRC), however, its regulation and functions are not fully understood. It has been shown that MUC13 protects colonic epithelial cells from apoptosis. Therefore, studying MUC13 and MUC13-regulated pathways may reveal promising therapeutic approaches for CRC treatment. Growing evidence suggests that microRNAs (miRs) are involved in the development and progression of CRC. In the present study, the MUC13-miR-4647 axis was addressed in association with survival of patients. miR-4647 is predicted in silico to bind to the MUC13 gene and was analyzed by RT-qPCR in 187 tumors and their adjacent non-malignant mucosa of patients with CRC. The impact of previously mentioned genes on survival and migration abilities of cancer cells was validated in vitro. Significantly upregulated MUC13 (P=0.02) in was observed tumor tissues compared with non-malignant adjacent mucosa, while miR-4647 (P=0.05) showed an opposite trend. Higher expression levels of MUC13 (log-rank P=0.05) were associated with worse patient's survival. The ectopic overexpression of studied miR resulted in decreased migratory abilities and worse survival of cells. Attenuated MUC13 expression levels confirmed the suppression of colony forming of CRC cells. In summary, the present data suggested the essential role of MUC13-miR-4647 in patients' survival, and this axis may serve as a novel therapeutic target. It is anticipated MUC13 may hold significant potential in the screening, diagnosis and treatment of CRC. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2024 Electronic address https://www.spandidos-publications.com/10.3892/ol.2022.13658
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