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Biological Auto(chemi)luminescence Imaging of Oxidative Processes in Human Skin
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SYSNO ASEP 0577023 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biological Auto(chemi)luminescence Imaging of Oxidative Processes in Human Skin Author(s) Poplová, Michaela (URE-Y)
Prasad, A. (IN)
van Wijk, E.P.A. (NL)
Pospíšil, P. (CZ)
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 5 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 95, č. 40 (2023), s. 14853-14860Number of pages 8 s. Publication form Print - P Language eng - English Country US - United States Keywords Luminescence ; Stress-induced ; Oxidative stress Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects GX20-06873X GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support URE-Y - RVO:67985882 UT WOS 001077073000001 EID SCOPUS 85174818928 DOI 10.1021/acs.analchem.3c01566 Annotation Oxidative processes in all types of organisms cause the chemical formation of electronically excited species, with subsequent ultraweak photon emission termed biological auto(chemi)luminescence (BAL). Imaging this luminescence phenomenon using ultrasensitive devices could potentially enable monitoring of oxidative stress in optically accessible areas of the human body, such as skin. Although oxidative stress induced by UV light has been explored, for chemically induced stress, there is no in vivo-quantified imaging of oxidative processes in human skin using BAL under the controlled extent of oxidative stress conditions. Furthermore, the mechanisms and dynamics of BAL from the skin have not been fully explored. Here, we demonstrate that different degrees of chemically induced oxidative stress on the skin can be spatially resolved quantitatively through noninvasive label-free BAL imaging. Additionally, to gain insight into the underlying mechanisms, a minimal chemical model of skin based on a mixture of lipid, melanin, and water was developed and used to show that it can be used to reproduce essential features of the response of real skin to oxidative stress. Our results contribute to novel, noninvasive photonic label-free methods for quantitative sensing of oxidative processes and oxidative stress. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2024 Electronic address DOI10.1021/acs.analchem.3c01566
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