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Cell lysis in the microfluidic chip: Towards single-molecule analysis in a free solution
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SYSNO ASEP 0576907 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Cell lysis in the microfluidic chip: Towards single-molecule analysis in a free solution Author(s) Křivánková, Jana (UIACH-O) RID, ORCID
Hlaváček, Antonín (UIACH-O) ORCID
Weisová, Julie (UIACH-O)Number of authors 3 Source Title 12th International Conference Analytical Cytometry : Book of abstracts. - Praha : AMCA, spol. s. r. o., 2023
S. 1-1Number of pages 1 s. Publication form Online - E Action International Conference Analytical Cytometry /12./ Event date 02.09.2023 - 05.09.2023 VEvent location Praha Country CZ - Czech Republic Event type CST Language eng - English Country CZ - Czech Republic Keywords droplet microfluidics ; HeLa cells ; detergent Triton X-100 Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA21-03156S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation Cell staining with Propidium iodide offered an effective tool for monitoring the progress of the cell lysis. Complete lysis in the microplate was reached with 0.01% (v/v) of the Triton X-100 after 10 minutes of passive detergent diffusion while 0.1% (v/v) of detergent was used for cell lysis in the PDMS droplet-based microfluidic chip. The proposed approach can be advantageously used for further cell manipulation and cell storage as well. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2024 Electronic address https://www.conference.csac.cz/Amca-CSAC/media/content/2023/docs/Book_of_abstracts_CSAC_2023.pdf
Number of the records: 1