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Ultrastructure and 3D reconstruction of a diplonemid protist (Diplonemea) and its novel membranous organelle
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SYSNO ASEP 0576570 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ultrastructure and 3D reconstruction of a diplonemid protist (Diplonemea) and its novel membranous organelle Author(s) Tashyreva, Daria (BC-A) RID, ORCID
Týč, Jiří (BC-A) ORCID, RID
Horák, Aleš (BC-A) RID, ORCID
Lukeš, Julius (BC-A) RID, ORCIDNumber of authors 4 Article number e0192123 Source Title mBio. - : American Society for Microbiology - ISSN 2161-2129
Roč. 14, SEP (2023)Number of pages 24 s. Publication form Online - E Language eng - English Country US - United States Keywords Euglenozoa ; cell division ; ultrastructure ; 3-dimensional reconstruction ; diplonemid ; sbf-sem Subject RIV EA - Cell Biology OECD category Cell biology R&D Projects GX23-06479X GA ČR - Czech Science Foundation (CSF) GA21-26209S GA ČR - Czech Science Foundation (CSF) LM2023050 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 001073880200001 EID SCOPUS 85176550181 DOI 10.1128/mbio.01921-23 Annotation Although diplonemid protists (Diplonemea, Euglenozoa) are among the most species-rich microeukaryotes in the ocean, many ultrastructural features of this lineage remain to be clarified. Using serial block-face scanning and transmission electron microscopy, we provide the whole-cell three-dimensional model of a previously undescribed diplonemid Lacrimia vacuolata sp. nov. For the first time, we were able to reconstruct a diplonemid cell in detail, including all its organelles and the flagellar and feeding apparatuses. By analyzing various stages of the cell cycle, we provide the first description of a diplonemid cell division, which is characterized by the disassembly of flagellar axonemes and the feeding apparatus and their further construction in the daughter cells. Moreover, we have identified a novel, ultrastructurally complex organelle, herein named the colv (Center for Organization of Layered Vesicles). We suggest that the colv is involved in food processing and membrane trafficking, and describe its close association with other components of the cellular digestive system.IMPORTANCE The knowledge of cell biology of a eukaryotic group is essential for correct interpretation of ecological and molecular data. Although diplonemid protists are one of the most species-rich lineages of marine eukaryotes, only very fragmentary information is available about the cellular architecture of this taxonomically diverse group. Here, a large serial block-face scanning electron microscopy data set complemented with light and fluorescence microscopy allowed the first detailed three-dimensional reconstruction of a diplonemid species. We describe numerous previously unknown peculiarities of the cellular architecture and cell division characteristic for diplonemid flagellates, and illustrate the obtained results with multiple three-dimensional models, comprehensible for non-specialists in protist ultrastructure. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://journals.asm.org/doi/10.1128/mbio.01921-23
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