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Light-induced damage to DNA origami nanostructures in the 193 nm-310 nm range
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SYSNO ASEP 0575871 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Light-induced damage to DNA origami nanostructures in the 193 nm-310 nm range Author(s) Sala, Leo Albert (UFCH-W) ORCID
Rakovský, Jozef (UFCH-W) RID, ORCID
Zerolova, A. (CZ)
Kočišek, Jaroslav (UFCH-W) RID, ORCIDArticle number 185101 Source Title Journal of Physics B-Atomic Molecular and Optical Physics. - : Institute of Physics Publishing - ISSN 0953-4075
Roč. 56, č. 18 (2023)Number of pages 8 s. Language eng - English Country US - United States Keywords DNA origami ; light ; uv ; damage ; DNA damage Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GX21-26601X GA ČR - Czech Science Foundation (CSF) EF16_026/0008382 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UFCH-W - RVO:61388955 UT WOS 001059517700001 EID SCOPUS 85171023334 DOI https://doi.org/10.1088/1361-6455/acf3bd Annotation DNA origami nanostructures provide precisely addressable substrates for in singulo experiments as well as for applications in nanotechnology. We report on experiments evaluating the stability of DNA origami upon irradiation with light at different wavelengths and buffer solutions. DNA is irradiated with nanosecond pulsed lasers and the damage is evaluated using UV-Vis spectroscopy and atomic force microscopy imaging. We show that the wavelength dependence of the damage follows the UV absorption spectrum of DNA. Electronic excitation of DNA is primarily responsible for DNA origami damage at present wavelengths. We also demonstrate UV-Vis absorption of tris reaction products, influencing the UV-Vis absorption evaluation in experiments studying DNA damage. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2024 Electronic address https://hdl.handle.net/11104/0345575
Number of the records: 1