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Cyanobacterial Harmful Bloom Lipopolysaccharides Induce Pro-Inflammatory Effects in Immune and Intestinal Epithelial Cells In Vitro

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    SYSNO ASEP0575765
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleCyanobacterial Harmful Bloom Lipopolysaccharides Induce Pro-Inflammatory Effects in Immune and Intestinal Epithelial Cells In Vitro
    Author(s) Skočková, Veronika (BFU-R)
    Vašíček, Ondřej (BFU-R) ORCID, RID
    Sychrová, E. (CZ)
    Sovadinová, I. (CZ)
    Babica, Pavel (BU-J) RID, ORCID
    Šindlerová, Lenka (BFU-R) RID, ORCID
    Number of authors6
    Article number169
    Source TitleToxins. - : MDPI - ISSN 2072-6651
    Roč. 15, č. 3 (2023)
    Number of pages19 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordscyanobacteria ; cyanobacterial harmful bloom ; lipopolysaccharide ; inflammation ; intestine ; macrophage
    Subject RIVFP - Other Medical Disciplines
    OECD categoryToxicology
    Subject RIV - cooperationInstitute of Botany - Other Medical Disciplines
    R&D ProjectsGA19-09980S GA ČR - Czech Science Foundation (CSF)
    Method of publishingOpen access
    Institutional supportBFU-R - RVO:68081707 ; BU-J - RVO:67985939
    UT WOS000959008100001
    EID SCOPUS85160553334
    DOI https://doi.org/10.3390/toxins15030169
    AnnotationFreshwater cyanobacterial harmful blooms (CyanoHABs) produce a variety of toxic and bioactive compounds including lipopolysaccharides (LPSs). The gastrointestinal tract can be exposed to them via contaminated water even during recreational activities. However, there is no evidence of an effect of CyanoHAB LPSs on intestinal cells. We isolated LPSs of four CyanoHABs dominated by different cyanobacterial species and LPSs of four laboratory cultures representing the respective dominant cyanobacterial genera. Two intestinal and one macrophage cell lines were used to detect in vitro pro-inflammatory activity of the LPS. All LPSs isolated from CyanoHABs and laboratory cultures induced cytokines production in at least one in vitro model, except for LPSs from the Microcystis PCC7806 culture. LPSs isolated from cyanobacteria showed unique migration patterns in SDS-PAGE that were qualitatively distinct from those of endotoxins from Gram-negative bacteria. There was no clear relationship between the biological activity of the LPS and the share of genomic DNA of Gram-negative bacteria in the respective biomass. Thus, the total share of Gram-negative bacteria, or the presence of Escherichia coli-like LPSs, did not explain the observed pro-inflammatory activities. The pro-inflammatory properties of environmental mixtures of LPSs from CyanoHABs indicate their human health hazards, and further attention should be given to their assessment and monitoring.
    WorkplaceInstitute of Biophysics
    ContactJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Year of Publishing2024
    Electronic addresshttps://www.mdpi.com/2072-6651/15/3/169
Number of the records: 1  

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