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Non-Rabl chromosome organization in endoreduplicated nuclei of barley embryo and endosperm tissues
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SYSNO ASEP 0575646 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Non-Rabl chromosome organization in endoreduplicated nuclei of barley embryo and endosperm tissues Author(s) Nowicka, Anna (UEB-Q) ORCID
Ferková, Ľuboslava (UEB-Q) RID
Said, Mahmoud (UEB-Q) ORCID
Kováčik, Martin (UEB-Q) ORCID
Zwyrtková, Jana (UEB-Q) ORCID
Baroux, C. (CH)
Pečinka, Aleš (UEB-Q) ORCID, RIDNumber of authors 7 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 74, č. 8 (2023), s. 2527-2541Number of pages 15 s. Language eng - English Country US - United States Keywords Barley ; cenh3 ; CEREBA repeat ; embryo ; endoreduplication ; endosperm ; Hordeum vulgare ; nuclear organization ; Rabl configuration OECD category Plant sciences, botany R&D Projects GA18-12197S GA ČR - Czech Science Foundation (CSF) GA21-02929S GA ČR - Czech Science Foundation (CSF) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000937763500001 EID SCOPUS 85159953310 DOI 10.1093/jxb/erad036 Annotation Rabl organization is a type of interphase chromosome arrangement with centromeres and telomeres clustering at opposite nuclear poles. Here, we analyzed nuclear morphology and chromosome organization in cycling and endoreduplicated nuclei isolated from embryo and endosperm tissues of developing barley seeds. We show that endoreduplicated nuclei have an irregular shape, less sister chromatid cohesion at 5S rDNA loci, and a reduced amount of centromeric histone CENH3. While the chromosomes of the embryo and endosperm nuclei are initially organized in Rabl configuration, the centromeres and telomeres are intermingled within the nuclear space in the endoreduplicated nuclei with an increasing endoreduplication level. Such a loss of chromosome organization suggests that Rabl configuration is introduced and further reinforced by mitotic divisions in barley cell nuclei in a tissue- and seed age-dependent manner. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1093/jxb/erad036
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