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TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance
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SYSNO ASEP 0575631 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance Author(s) Wang, J. (BE)
Jiang, Q. (BE)
Pleskot, Roman (UEB-Q) RID, ORCID
Grones, P. (BE)
Bahafid, E. (DE)
Denay, G. (DE)
Galván-Ampudia, C. (FR)
Xu, X. (CN)
Vandorpe, M. (BE)
Mylle, E. (BE)
De Smet, I. (BE)
Vernoux, T. (FR)
Simon, R. S. (DE)
Nowack, M.K. (BE)
Van Damme, D. (BE)Number of authors 15 Article number e54709 Source Title Embo Reports - ISSN 1469-221X
Roč. 24, č. 9 (2023)Number of pages 13 s. Language eng - English Country US - United States Keywords clathrin-mediated endocytosis ; CLAVATA3-CLAVATA1 signaling ; shoot apical meristem maintenance ; TPLATE complex OECD category Cell biology Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 001029673900001 EID SCOPUS 85164917625 DOI https://doi.org/10.15252/embr.202254709 Annotation Endocytosis regulates the turnover of cell surface localized receptors, which are crucial for plants to rapidly respond to stimuli. The evolutionary ancient TPLATE complex (TPC) plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of single TPC subunits causes male sterility and seedling lethality phenotypes, complicating analysis of the roles of TPC during plant development. Partially functional alleles of TPC subunits however only cause mild developmental deviations. Here, we took advantage of the partially functional TPLATE allele, WDXM2, to investigate a role for TPC-dependent endocytosis in receptor-mediated signaling. We discovered that reduced TPC-dependent endocytosis confers a hypersensitivity to very low doses of CLAVATA3 peptide signaling. This hypersensitivity correlated with the abundance of the CLAVATA3 receptor protein kinase CLAVATA1 at the plasma membrane. Genetic and biochemical analysis as well as live-cell imaging revealed that TPC-dependent regulation of CLAVATA3-dependent internalization of CLAVATA1 from the plasma membrane is required for shoot stem cell homeostasis. Our findings provide evidence that TPC-mediated endocytosis and degradation of CLAVATA1 is a mechanism to dampen CLAVATA3-mediated signaling during plant development. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.15252/embr.202254709
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