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TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance
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SYSNO ASEP 0575631 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance Tvůrce(i) Wang, J. (BE)
Jiang, Q. (BE)
Pleskot, Roman (UEB-Q) RID, ORCID
Grones, P. (BE)
Bahafid, E. (DE)
Denay, G. (DE)
Galván-Ampudia, C. (FR)
Xu, X. (CN)
Vandorpe, M. (BE)
Mylle, E. (BE)
De Smet, I. (BE)
Vernoux, T. (FR)
Simon, R. S. (DE)
Nowack, M.K. (BE)
Van Damme, D. (BE)Celkový počet autorů 15 Číslo článku e54709 Zdroj.dok. Embo Reports - ISSN 1469-221X
Roč. 24, č. 9 (2023)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova clathrin-mediated endocytosis ; CLAVATA3-CLAVATA1 signaling ; shoot apical meristem maintenance ; TPLATE complex Obor OECD Cell biology Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 001029673900001 EID SCOPUS 85164917625 DOI https://doi.org/10.15252/embr.202254709 Anotace Endocytosis regulates the turnover of cell surface localized receptors, which are crucial for plants to rapidly respond to stimuli. The evolutionary ancient TPLATE complex (TPC) plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of single TPC subunits causes male sterility and seedling lethality phenotypes, complicating analysis of the roles of TPC during plant development. Partially functional alleles of TPC subunits however only cause mild developmental deviations. Here, we took advantage of the partially functional TPLATE allele, WDXM2, to investigate a role for TPC-dependent endocytosis in receptor-mediated signaling. We discovered that reduced TPC-dependent endocytosis confers a hypersensitivity to very low doses of CLAVATA3 peptide signaling. This hypersensitivity correlated with the abundance of the CLAVATA3 receptor protein kinase CLAVATA1 at the plasma membrane. Genetic and biochemical analysis as well as live-cell imaging revealed that TPC-dependent regulation of CLAVATA3-dependent internalization of CLAVATA1 from the plasma membrane is required for shoot stem cell homeostasis. Our findings provide evidence that TPC-mediated endocytosis and degradation of CLAVATA1 is a mechanism to dampen CLAVATA3-mediated signaling during plant development. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2024 Elektronická adresa https://doi.org/10.15252/embr.202254709
Number of the records: 1