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Solicola gregarius gen. nov., sp. nov., a soil actinobacterium isolated after enhanced cultivation with Micrococcus luteus culture supernatant
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SYSNO ASEP 0574682 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Solicola gregarius gen. nov., sp. nov., a soil actinobacterium isolated after enhanced cultivation with Micrococcus luteus culture supernatant Author(s) Lopez Marin, M. A. (CZ)
Šuman, J. (CZ)
Jani, K. (CZ)
Ulbrich, P. (CZ)
Cajthaml, Tomáš (MBU-M) RID, ORCID
Filipová, Alena (MBU-M) RID, ORCID
Pajer, P. (CZ)
Neumann-Schaal, M. (DE)
Strejček, M. (CZ)
Uhlík, O. (CZ)Article number 005678 Source Title International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026
Roč. 73, č. 1 (2023)Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords compost soil ; extracellular organic matter ; Nocardioidaceae ; resuscitation-promoting factor ; soil bacteria ; whole-genome average nucleotide identity OECD category Microbiology R&D Projects LM2018131 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 001008263300027 EID SCOPUS 85146775471 DOI https://doi.org/10.1099/ijsem.0.005678 Annotation An actinobacterial strain, designated A5X3R13(T), was isolated from a compost soil suspension supplemented with extracellular material from a Micrococcus luteus-culture supernatant. The strain was cultured on tenfold-diluted reasoner's 2A agar. The cells were ovoid-to-rod shaped, non-motile, Gram-stain-positive, oxidase-negative, catalase-positive and had a width of 0.5 mu m and a length of 0.8-1.2 mu m. The results of both 16S rRNA-based phylogenetic and whole-genome analyses indicate that A5X3R13(T) forms a distinct lineage within the family Nocardioidaceae (order Propionibacteriales). On the basis of the 16S rRNA gene sequence, A5X3R13(T) was closely related to Aeromicrobium terrae CC-CFT486T (96.2%), Nocardioides iriomotensis IR27-S3(T) (96.2%), Nocardioides guangzhouensis 130(T) (95.6%), Marmoricola caldifontis YIM 730233T (95.5 %), Aeromicrobium alkaliterrae KSL-107(T) (95.4%), Aeromicrobium choanae 9H-4T (95.4%), Aeromicrobium panaciterrae Gsoil 161(T) (95.3 %), and Nocardioides jensenii NBRC 14755(T) (95.2 %). The genome had a length of 4915 757 bp, and its DNA G+C content was 68.5 mol %. The main fatty acids were 10-methyl C-17:0, C-16:0, C-15:0, C-18:0, C-17:0 and iso-C-16:0. The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. MK-9(H 4) was the predominant respiratory quinone. The peptidoglycan type was A3 gamma (A41.1) and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid in a molar ratio of 1.2 :0.9:1.0:0.8. On the basis of the results of the phylogenetic and phenotypic analyses and comparisons with other members of the family Nocardioidaceae, strain A5X3R13(T) is proposed to represent a novel species within a novel genus, for which the name Solicola gregarius gen. nov., sp. nov. is proposed. The type strain is A5X3R13(T) (=DSM 112953(T)=NCCB 100840T). Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005678
Number of the records: 1