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Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding
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SYSNO ASEP 0574364 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Thermoneutral housing promotes hepatic steatosis in standard diet-fed C57BL/6N mice, with a less pronounced effect on NAFLD progression upon high-fat feeding Author(s) Horáková, Olga (FGU-C) RID, ORCID
Sistilli, Gabriella (FGU-C)
Kalendová, Veronika (FGU-C) RID, ORCID
Bardová, Kristina (FGU-C) RID, ORCID, SAI
Mitrović, Marko (FGU-C)
Čajka, Tomáš (FGU-C) RID, ORCID, SAI
Irodenko, Ilaria (FGU-C)
Janovská, Petra (FGU-C) RID, ORCID
Lackner, K. (AT)
Kopecký, Jan (FGU-C) RID, ORCID
Rossmeisl, Martin (FGU-C) RID, ORCIDArticle number 1205703 Source Title Frontiers in Endocrinology. - : Frontiers Media - ISSN 1664-2392
Roč. 14, Jul 12 (2023)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords non-alcoholic fatty liver disease ; liver steatosis ; NASH ; C57BL/6N mice ; obesity ; thermoneutrality ; metabolomics ; de novo lipogenesis OECD category Endocrinology and metabolism (including diabetes, hormones) R&D Projects NU21-01-00469 GA MZd - Ministry of Health (MZ) LX22NPO5104 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 001035615900001 EID SCOPUS 85165956646 DOI 10.3389/fendo.2023.1205703 Annotation Introduction: Non-alcoholic fatty liver disease (NAFLD) can progress to more severe stages, such as steatohepatitis and fibrosis. Thermoneutral housing together with high-fat diet promoted NAFLD progression in C57BL/6J mice. Due to possible differences in steatohepatitis development between different C57BL/6 substrains, we examined how thermoneutrality affects NAFLD progression in C57BL/6N mice.
Methods: Male mice were fed standard or high-fat diet for 24 weeks and housed under standard (22°C) or thermoneutral (30°C) conditions.
Results: High-fat feeding promoted weight gain and hepatic steatosis, but the effect of thermoneutral environment was not evident. Liver expression of inflammatory markers was increased, with a modest and inconsistent effect of thermoneutral housing, however, histological scores of inflammation and fibrosis were generally low (<1.0), regardless of ambient temperature. In standard diet-fed mice, thermoneutrality increased weight gain, adiposity, and hepatic steatosis, accompanied by elevated de novo lipogenesis and changes in liver metabolome characterized by complex decreases in phospholipids and metabolites involved in urea cycle and oxidative stress defense.
Conclusion: Thermoneutrality appears to promote NAFLD-associated phenotypes depending on the C57BL/6 substrain and/or the amount of dietary fat.
Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2024 Electronic address https://doi.org/10.3389/fendo.2023.1205703
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