3D-printed device for blood DNA fractionation

0573261 - ÚIACH 2024 DE eng A - Abstract
Hrušková, Helena - Řemínek, Roman - Foret, František
3D-printed device for blood DNA fractionation.
HPLC 2023. Final Program. Frankfurt am Main: Gesellschaft Deutscher Chemiker e.V., 2023 - (Lämmerhofer, M.; Schmitz, O.), č. článku P-CEMI13.
[International Symposium on High-Performance Liquid Phase Separations and Related Techniques (HPLC 2023) /51./. 18.06.2023-22.06.2023, Duesseldorf]
Institutional support: RVO:68081715
Keywords : blood plasma * DNA fragments * DNA fractionation
OECD category: Analytical chemistry

In our research, we focused on the improvement of the sensitivity of PCR detection of short tumor DNA fragments. For that purpose, we decided to preclean blood samples off large DNA fragments that are not usable as biomarkers. For sample precleaning, we have developed a new 3D-printed device, that allows DNA fractionation. The device is fabricated from chemically and mechanically stable HD-PLA. The precleaning technique is based on gel electromigration separation techniques. A method for the separation of short DNA fragments was developed, partially validated, and tested on blood plasma and serum matrices.
Permanent Link: https://hdl.handle.net/11104/0343731