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Identification of silk components in the bombycoid moth Andraca theae (Endromidae) reveals three fibroin subunits resembling those of Bombycidae and Sphingidae

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    0572250 - BC 2024 RIV GB eng J - Journal Article
    Kmeť, Peter - Kučerová, Lucie - Sehadová, Hana - Wu, Chia-hsiang - Wu, Y.-L. - Žurovec, Michal
    Identification of silk components in the bombycoid moth Andraca theae (Endromidae) reveals three fibroin subunits resembling those of Bombycidae and Sphingidae.
    Journal of Insect Physiology. Roč. 147, JUN 01 (2023), č. článku 104523. ISSN 0022-1910. E-ISSN 1879-1611
    R&D Projects: GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF16_013/0001775
    Grant - others:GA MŠk(CZ) CZ.1.05/1.1.00/02.0109; Interreg Bayern Tschechische Republik(AT) Ziel ETZ2021-2022, no. 331
    Institutional support: RVO:60077344
    Keywords : zonadhesin-like * adhesives * sericin
    OECD category: Biochemistry and molecular biology
    Impact factor: 2.2, year: 2022
    Method of publishing: Limited access
    https://www.sciencedirect.com/science/article/pii/S0022191023000495/pdfft?md5=1611d6411fdd9b4f209abe959882eb5f&pid=1-s2.0-S0022191023000495-main.pdf

    The silk produced by Lepidoptera caterpillars is a mixture of proteins secreted by the transformed labial glands, the silk glands (SG). The silk fiber consists of insoluble filamentous proteins that form a silk core and are produced in the posterior part of the SG and soluble coat proteins consisting of sericins and various other polypeptides secreted in the middle part of the SG. We constructed a silk gland specific transcriptome of Andraca theae and created a protein database required for peptide mass fingerprinting. We identified major silk components by proteomic analysis of cocoon silk and by searching for homologies with known silk protein sequences from other species. We identified 30 proteins including a heavy chain fibroin, a light chain fibroin and fibrohexamerin (P25) that form the silk core, as well as members of several structural families that form the silk coating. To uncover the evolutionary relationships among silk proteins, we included orthologs of silk genes from several recent genome projects and performed phylogenetic analyses. Our results confirm the recent molecular classification that the family Endromidae appears to be slightly more distant from the family Bombycidae. Our study provides important information on the evolution of silk proteins in the Bombycoidea, which is needed for proper annotation of the proteins and future functional studies.
    Permanent Link: https://hdl.handle.net/11104/0349841

     
     
Number of the records: 1  

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