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Transcriptomic hallmarks of in vitro TiOinf2/inf nanotubes toxicity in Chlamydomonas reinhardtii
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SYSNO ASEP 0571826 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptomic hallmarks of in vitro TiOinf2/inf nanotubes toxicity in Chlamydomonas reinhardtii Author(s) Dobešová, M. (CZ)
Koláčková, M. (CZ)
Pencik, O. (CZ)
Cápal, Petr (UEB-Q) RID, ORCID
Chaloupsky, P. (CZ)
Švec, P. (CZ)
Ridošková, A. (CZ)
Motola, M. (CZ)
Cicmancova, V. (CZ)
Sopha, H. (CZ)
Macák, J. M. (CZ)
Richtera, L. (CZ)
Adam, V. (CZ)
Húska, D. (CZ)Number of authors 14 Article number 106419 Source Title Aquatic Toxicology. - : Elsevier - ISSN 0166-445X
Roč. 256, MAR (2023)Number of pages 14 s. Language eng - English Country NL - Netherlands Keywords Chlamydomonas ; Nanotubes ; RNA-seq ; TiO 2 ; Toxicity OECD category Genetics and heredity (medical genetics to be 3) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000948303100001 EID SCOPUS 85148356897 DOI 10.1016/j.aquatox.2023.106419 Annotation Recently, more accessible transcriptomic approaches have provided a new and deeper understanding of environmental toxicity. The present study focuses on the transcriptomic profiles of green microalgae Chlamydomonas reinhardtii exposed to new industrially promising material, TiO2 nanotubes (NTs), as an example of a widely used one-dimensional nanomaterial. The first algal in vitro assay included 2.5 and 7.5 mg/L TiO2 NTs, resulting in a dose-dependent negative effect on biological endpoints. At a working concentration of 7.5 mg/L, RNA-sequencing showed a mainly negative effect on the cells. In summary, the results indicated metabolic disruption, such as ATP loss, damage to mitochondria and chloroplasts, loss of solutes due to permeated membranes, and cell wall damage. Moreover, apoptosis-induced transcripts were detected. Interestingly, reactivation of transposons was observed. In signalling and transcription pathways, including chromatin remodelling and locking, the annotated genes were downregulated. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1016/j.aquatox.2023.106419
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