Number of the records: 1  

Short-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics

  1. 1.
    SYSNO ASEP0571360
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleShort-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics
    Author(s) Hricko, Jiří (FGU-C)
    Rudl Kulhavá, Lucie (FGU-C) ORCID, RID
    Paučová, Michaela (FGU-C)
    Nováková, Michaela (FGU-C)
    Kuda, Ondřej (FGU-C) RID, ORCID, SAI
    Fiehn, O. (US)
    Čajka, Tomáš (FGU-C) RID, ORCID, SAI
    Article number986
    Source TitleAntioxidants. - : MDPI
    Roč. 12, č. 5 (2023)
    Number of pages15 s.
    Languageeng - English
    CountryCH - Switzerland
    Keywordsmetabolomics ; lipidomics ; stability ; shipping ; oxidation ; serum ; liver ; tissue ; liquid chromatography ; mass spectrometry ; LC-MS
    OECD categoryEndocrinology and metabolism (including diabetes, hormones)
    R&D ProjectsLX22NPO5104 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    NU20-01-00186 GA MZd - Ministry of Health (MZ)
    NU22-02-00161 GA MZd - Ministry of Health (MZ)
    GA21-00477S GA ČR - Czech Science Foundation (CSF)
    LTAUSA19124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Research Infrastructuree-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob
    Method of publishingOpen access
    Institutional supportFGU-C - RVO:67985823
    UT WOS000995665700001
    EID SCOPUS85160639573
    DOI10.3390/antiox12050986
    AnnotationThermal reactions can significantly alter the metabolomic and lipidomic content of biofluids and tissues during storage. In this study, we investigated the stability of polar metabolites and complex lipids in dry human serum and mouse liver extracts over a three-day period under various temperature conditions. Specifically, we tested temperatures of −80 °C (freezer), −24 °C (freezer), −0.5 °C (polystyrene box with gel-based ice packs), +5 °C (refrigerator), +23 °C (laboratory, room temperature), and +30 °C (thermostat) to simulate the time between sample extraction and analysis, shipping dry extracts to different labs as an alternative to dry ice, and document the impact of higher temperatures on sample integrity. The extracts were analyzed using five fast liquid chromatography-mass spectrometry (LC-MS) methods to screen polar metabolites and complex lipids, and over 600 metabolites were annotated in serum and liver extracts. We found that storing dry extracts at −24 °C and partially at −0.5 °C provided comparable results to −80 °C (reference condition). However, increasing the storage temperatures led to significant changes in oxidized triacylglycerols, phospholipids, and fatty acids within three days. Polar metabolites were mainly affected at storage temperatures of +23 °C and +30 °C.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Year of Publishing2024
    Electronic addresshttps://www.mdpi.com/2076-3921/12/5/986
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.