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Short-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics
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SYSNO ASEP 0571360 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Short-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics Author(s) Hricko, Jiří (FGU-C)
Rudl Kulhavá, Lucie (FGU-C) ORCID, RID
Paučová, Michaela (FGU-C)
Nováková, Michaela (FGU-C)
Kuda, Ondřej (FGU-C) RID, ORCID, SAI
Fiehn, O. (US)
Čajka, Tomáš (FGU-C) RID, ORCID, SAIArticle number 986 Source Title Antioxidants. - : MDPI
Roč. 12, č. 5 (2023)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords metabolomics ; lipidomics ; stability ; shipping ; oxidation ; serum ; liver ; tissue ; liquid chromatography ; mass spectrometry ; LC-MS OECD category Endocrinology and metabolism (including diabetes, hormones) R&D Projects LX22NPO5104 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NU20-01-00186 GA MZd - Ministry of Health (MZ) NU22-02-00161 GA MZd - Ministry of Health (MZ) GA21-00477S GA ČR - Czech Science Foundation (CSF) LTAUSA19124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000995665700001 EID SCOPUS 85160639573 DOI 10.3390/antiox12050986 Annotation Thermal reactions can significantly alter the metabolomic and lipidomic content of biofluids and tissues during storage. In this study, we investigated the stability of polar metabolites and complex lipids in dry human serum and mouse liver extracts over a three-day period under various temperature conditions. Specifically, we tested temperatures of −80 °C (freezer), −24 °C (freezer), −0.5 °C (polystyrene box with gel-based ice packs), +5 °C (refrigerator), +23 °C (laboratory, room temperature), and +30 °C (thermostat) to simulate the time between sample extraction and analysis, shipping dry extracts to different labs as an alternative to dry ice, and document the impact of higher temperatures on sample integrity. The extracts were analyzed using five fast liquid chromatography-mass spectrometry (LC-MS) methods to screen polar metabolites and complex lipids, and over 600 metabolites were annotated in serum and liver extracts. We found that storing dry extracts at −24 °C and partially at −0.5 °C provided comparable results to −80 °C (reference condition). However, increasing the storage temperatures led to significant changes in oxidized triacylglycerols, phospholipids, and fatty acids within three days. Polar metabolites were mainly affected at storage temperatures of +23 °C and +30 °C. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2024 Electronic address https://www.mdpi.com/2076-3921/12/5/986
Number of the records: 1