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Short-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics
- 1.0571360 - FGÚ 2024 RIV CH eng J - Journal Article
Hricko, Jiří - Rudl Kulhavá, Lucie - Paučová, Michaela - Nováková, Michaela - Kuda, Ondřej - Fiehn, O. - Čajka, Tomáš
Short-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics.
Antioxidants. Roč. 12, č. 5 (2023), č. článku 986. E-ISSN 2076-3921
R&D Projects: GA MŠMT(CZ) LX22NPO5104; GA MZd(CZ) NU20-01-00186; GA MZd(CZ) NU22-02-00161; GA ČR(CZ) GA21-00477S; GA MŠMT(CZ) LTAUSA19124
Research Infrastructure: e-INFRA CZ - 90140
Institutional support: RVO:67985823
Keywords : metabolomics * lipidomics * stability * shipping * oxidation * serum * liver * tissue * liquid chromatography * mass spectrometry * LC-MS
OECD category: Endocrinology and metabolism (including diabetes, hormones)
Impact factor: 6, year: 2023
Method of publishing: Open access
https://www.mdpi.com/2076-3921/12/5/986
Thermal reactions can significantly alter the metabolomic and lipidomic content of biofluids and tissues during storage. In this study, we investigated the stability of polar metabolites and complex lipids in dry human serum and mouse liver extracts over a three-day period under various temperature conditions. Specifically, we tested temperatures of −80 °C (freezer), −24 °C (freezer), −0.5 °C (polystyrene box with gel-based ice packs), +5 °C (refrigerator), +23 °C (laboratory, room temperature), and +30 °C (thermostat) to simulate the time between sample extraction and analysis, shipping dry extracts to different labs as an alternative to dry ice, and document the impact of higher temperatures on sample integrity. The extracts were analyzed using five fast liquid chromatography-mass spectrometry (LC-MS) methods to screen polar metabolites and complex lipids, and over 600 metabolites were annotated in serum and liver extracts. We found that storing dry extracts at −24 °C and partially at −0.5 °C provided comparable results to −80 °C (reference condition). However, increasing the storage temperatures led to significant changes in oxidized triacylglycerols, phospholipids, and fatty acids within three days. Polar metabolites were mainly affected at storage temperatures of +23 °C and +30 °C.
Permanent Link: https://hdl.handle.net/11104/0342602
File Download Size Commentary Version Access 23_0029_0571360.pdf 4 2.2 MB Publisher’s postprint open-access
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