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Singlet oxygen in vivo: it is all about intensity - part 2
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SYSNO ASEP 0571287 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Singlet oxygen in vivo: it is all about intensity - part 2 Author(s) Hackbarth, S. (DE)
Gao, S. (JP)
Šubr, Vladimír (UMCH-V) RID, ORCID
Lin, L. (DE)
Pohl, J. (DE)
Etrych, Tomáš (UMCH-V) RID, ORCID
Fang, J. (JP)Article number 781 Source Title Journal of Personalized Medicine. - : MDPI
Roč. 13, č. 5 (2023)Number of pages 14 s. Language eng - English Country CH - Switzerland Keywords photodynamic therapy ; singlet oxygen ; time-resolved phosphorescence Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects NU21-08-00280 GA MZd - Ministry of Health (MZ) LX22NPO5102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMCH-V - RVO:61389013 UT WOS 001020900500001 EID SCOPUS 85160331617 DOI 10.3390/jpm13050781 Annotation Recently, we reported induced anoxia as a limiting factor for photodynamic tumor therapy (PDT). This effect occurs in vivo if the amount of generated singlet oxygen that undergoes chemical reactions with cellular components exceeds the local oxygen supply. The amount of generated singlet oxygen depends mainly on photosensitizer (PS) accumulation, efficiency, and illumination intensity. With illumination intensities above a certain threshold, singlet oxygen is limited to the blood vessel and the nearest vicinity. Lower intensities allow singlet oxygen generation also in tissue which is a few cell layers away from the vessels. While all experiments so far were limited to light intensities above this threshold, we report experimental results for intensities at both sides of the threshold for the first time, giving proof for the described model. Using time-resolved optical detection in NIR, we demonstrate characteristic, illumination intensity-dependent changes in signal kinetics of singlet oxygen and photosensitizer phosphorescence in vivo. The described analysis allows for better optimization and coordination of PDT drugs and treatment, as well as new diagnostic methods based on gated PS phosphorescence, for which we report a first in vivo feasibility test.
Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2024 Electronic address https://www.mdpi.com/2075-4426/13/5/781
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