Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology

Filipi, Tereza; Matúšová, Zuzana; Abaffy, Pavel; Vaňátko, Ondřej; Turečková, Jana; Benešová, Šárka; Kubísková, Monika; Kirdajová, Denisa; Zahumenský, Jakub; Valihrach, Lukáš; Anděrová, Miroslava

doi:https://dx.doi.org/10.1038/s41598-023-33608-y

Number of the records: 1

Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology

1.

SYSNO ASEP	0571245
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology
Author(s)	Filipi, Tereza (UEM-P) Matúšová, Zuzana (BTO-N) Abaffy, Pavel (BTO-N) Vaňátko, Ondřej (UEM-P) Turečková, Jana (UEM-P)_ORCID Benešová, Šárka (BTO-N) Kubísková, Monika (UEM-P) Kirdajová, Denisa (UEM-P)_{RID, ORCID} Zahumenský, Jakub (UEM-P)_{ORCID, RID} Valihrach, Lukáš (BTO-N)_{RID, ORCID} Anděrová, Miroslava (UEM-P)_{RID, ORCID}
Article number	6538
Source Title	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 13, april (2023)
Number of pages	15 s.
Language	eng - English
Country	US - United States
Keywords	amyotrophic-lateral-sclerosis ; motor-neuron degeneration ; transgenic mouse model ; differential expressiongene-expression ; spinal-cord ; microglia ; disease ; astrocytes ; activation
OECD category	Neurosciences (including psychophysiology
R&D Projects	GA23-05327S GA ČR - Czech Science Foundation (CSF)
	GA19-02046S GA ČR - Czech Science Foundation (CSF)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LX22NPO5107 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2023050 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEM-P - RVO:68378041 ; BTO-N - RVO:86652036
UT WOS	000989734900016
EID SCOPUS	85153548229
DOI	10.1038/s41598-023-33608-y
Annotation	The role of glia in amyotrophic lateral sclerosis (ALS) is undeniable. Their disease-related activity has been extensively studied in the spinal cord, but only partly in the brain. We present herein a comprehensive study of glia in the cortex of SOD1(G93A) mice—a widely used model of ALS. Using single-cell RNA sequencing (scRNA-seq) and immunohistochemistry, we inspected astrocytes, microglia, and oligodendrocytes, in four stages of the disease, respecting the factor of sex. We report minimal changes of glia throughout the disease progression and regardless of sex. Pseudobulk and single-cell analyses revealed subtle disease-related transcriptional alterations at the end-stage in microglia and oligodendrocytes, which were supported by immunohistochemistry. Therefore, our data support the hypothesis that the SOD1(G93A) mouse cortex does not recapitulate the disease in patients, and we recommend the use of a different model for future studies of the cortical ALS pathology.
Workplace	Institute of Experimental Medicine
Contact	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2024
Electronic address	https://www.nature.com/articles/s41598-023-33608-y

Number of the records: 1