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Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs
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SYSNO ASEP 0569875 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Proteases and their inhibitors involved in Schistosoma mansoni egg-host interaction revealed by comparative transcriptomics with Fasciola hepatica eggs Author(s) Peterková, K. (CZ)
Vorel, J. (CZ)
Ilgová, J. (CZ)
Ostašov, P. (CZ)
Fajtová, Pavla (UOCHB-X) RID, ORCID
Konečný, L. (CZ)
Chanová, M. (CZ)
Kašný, M. (CZ)
Horn, Martin (UOCHB-X) RID, ORCID
Dvořák, Jan (UOCHB-X) ORCIDSource Title International Journal for Parasitology. - : Elsevier - ISSN 0020-7519
Roč. 53, 5/6 (2023), s. 253-263Number of pages 11 s. Language eng - English Country AU - Australia Keywords Schistosoma mansoni ; egg ; Fasciola hepatica ; comparative transcriptomics ; protease ; protease inhibitor OECD category Biochemistry and molecular biology R&D Projects LM2018131 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA19-17269S GA ČR - Czech Science Foundation (CSF) NV18-05-00345 GA MZd - Ministry of Health (MZ) Research Infrastructure e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 001002409800001 EID SCOPUS 85148365946 DOI 10.1016/j.ijpara.2022.12.007 Annotation Schistosoma mansoni eggs are the main causative agents of the pathological manifestations of schistosomiasis. The eggs are laid in the host bloodstream, then they migrate through the intestinal wall into the lumen. However, a significant proportion of the eggs become lodged in the liver, where they cause inflammation and fibrosis. In this study, we focus on a specific group of proteins expressed by the egg, namely proteases and their inhibitors. These molecules are often involved in schistosome-host interactions, but are still unexplored in the egg stage. Using RNA-seq and comparative transcriptomics of immature and mature S. mansoni eggs, we mapped the portfolio of proteases and their inhibitors, and determined their gene expression levels. In addition, we compared these data with gene expression of proteases and their inhibitors in Fasciola hepatica eggs. Fasciola hepatica eggs served as a useful comparative model, as they do not migrate through tissues and inflict pathology. We detected transcription of 135 and 117 proteases in S. mansoni and F. hepatica eggs, respectively, with 87 identified as orthologous between the two species. In contrast, we observed only four orthologous inhibitors out of 21 and 16 identified in S. mansoni and F. hepatica eggs, respectively. Among others, we measured high and developmentally regulated levels of expression of metalloproteases in S. mansoni eggs, specifically aminopeptidase N1, endothelin-converting enzyme 1, and several leishmanolysin-like peptidases. We identified highly transcribed protease inhibitors serpin and alpha-2-macroglobulin that are unique to S. mansoni eggs, and antistasin-like inhibitor in F. hepatica eggs. This study provides new insights into the portfolio of proteases and inhibitors expressed by S. mansoni with potential roles in egg tissue migration, stimulation of angiogenesis, and interaction with host blood and immunity. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2024 Electronic address https://doi.org/10.1016/j.ijpara.2022.12.007
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