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Arabidopsis thaliana NudiXes have RNA-decapping activity
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SYSNO ASEP 0569480 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Arabidopsis thaliana NudiXes have RNA-decapping activity Author(s) Mititelu, Maria Bianca (UOCHB-X)
Hudeček, Oldřich (UOCHB-X)
Gozdek, A. (PL)
Benoni, Roberto (UOCHB-X)
Nešuta, Ondřej (UOCHB-X) RID, ORCID
Krasnodebski, S. (PL)
Kufel, J. (PL)
Cahová, Hana (UOCHB-X) RID, ORCIDSource Title RSC Chemical Biology. - : Royal Society of Chemistry - ISSN 2633-0679
Roč. 4, č. 3 (2023), s. 223-228Number of pages 6 s. Language eng - English Country GB - United Kingdom Keywords hydrolases ; enzymes ; family OECD category Biochemistry and molecular biology R&D Projects LL1603 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UOCHB-X - RVO:61388963 UT WOS 000915348600001 EID SCOPUS 85150293104 DOI 10.1039/d2cb00213b Annotation Recent discoveries of various noncanonical RNA caps, such as dinucleoside polyphosphates (NpnN), coenzyme A (CoA), and nicotinamide adenine dinucleotide (NAD) in all domains of life have led to a revision of views on RNA cap function and metabolism. Enzymes from the NudiX family capable of hydrolyzing a polyphosphate backbone attached to a nucleoside are the strongest candidates for degradation of noncanonically capped RNA. The model plant organism Arabidopsis thaliana encodes as many as 28 NudiX enzymes. For most of them, only in vitro substrates in the form of small molecules are known. In our study, we focused on four A. thaliana NudiX enzymes (AtNUDT6, AtNUDT7, AtNUDT19 and AtNUDT27), and we studied whether these enzymes can cleave RNA capped with Np(n)Ns (Ap(2-5)A, Gp(3-4)G, Ap(3-5)G, m(7)Gp(3)G, m(7)Gp(3)A), CoA, ADP-ribose, or NAD(H). While AtNUDT19 preferred NADH-RNA over other types of capped RNA, AtNUDT6 and AtNUDT7 preferentially cleaved Ap(4)A-RNA. The most powerful decapping enzyme was AtNUDT27, which cleaved almost all types of capped RNA at a tenfold lower concentration than the other enzymes. We also compared cleavage efficiency of each enzyme on free small molecules with RNA capped with corresponding molecules. We found that AtNUDT6 prefers free Ap(4)A, while AtNUDT7 preferentially cleaved Ap(4)A-RNA. These findings show that NudiX enzymes may act as RNA-decapping enzymes in A. thaliana and that other noncanonical RNA caps such as Ap(4)A and NADH should be searched for in plant RNA. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2024 Electronic address https://doi.org/10.1039/D2CB00213B
Number of the records: 1