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Chemical Biopsy for GNMT as Noninvasive and Tumorigenesis-Relevant Diagnosis of Liver Cancer

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    SYSNO ASEP0568951
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleChemical Biopsy for GNMT as Noninvasive and Tumorigenesis-Relevant Diagnosis of Liver Cancer
    Author(s) Oh, S. (KR)
    Jo, S. (KR)
    Kim, H. S. (KR)
    Mai, V. (KR)
    Endaya, B. (AU)
    Neužil, Jiří (BTO-N) RID
    Jung, K. H. (KR)
    Hong, S. (KR)
    Kim, J. (KR)
    Park, S. (KR)
    Number of authors10
    Source TitleAnalytical Chemistry. - : American Chemical Society - ISSN 0003-2700
    Roč. 95, č. 2 (2023), s. 1184-1192
    Number of pages9 s.
    Languageeng - English
    CountryUS - United States
    KeywordsGLYCINE N-METHYLTRANSFERASE ; INFILTRATIVE HEPATOCELLULAR-CARCINOMA ; FOLATE-BINDING-PROTEIN
    Subject RIVCB - Analytical Chemistry, Separation
    OECD categoryAnalytical chemistry
    Method of publishingLimited access
    Institutional supportBTO-N - RVO:86652036
    UT WOS000923938900001
    EID SCOPUS85145985336
    DOI10.1021/acs.analchem.2c03944
    AnnotationEarly diagnosis of hepatocellular carcinoma (HCC) is difficult, the patients burdened by life-long periodical examinations. Here, a new chemical biopsy approach was developed for noninvasive diagnosis of HCC using urine samples. Bioinformatic screening for tumor suppressors yielded glycine Nmethyltransferase (GNMT) as a biomarker with clinical relevance to HCC tumorigenesis. A liquid chromatography-mass spectrometry (LC-MS)-based chemical biopsy detecting nonradioactive 13C-sarcosine from 13C-glycine was designed to noninvasively assess liver GNMT activity extrahepatically. 13CSarcosine showed a strong correlation with GNMT in normal and cancerous liver cells. In an autochthonous animal model developing visible cancer nodules at 17 weeks, the urinary 13C-sarcosine chemical biopsy exhibited notable changes as early as 8 weeks, showing significant correlations with liver GNMT and molecular pathological changes. Our chemical biopsy approach should facilitate early and noninvasive diagnosis of HCC, with direct relevance to tumorigenesis, which can be straightforwardly applied to other diseases.
    WorkplaceInstitute of Biotechnology
    ContactMonika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
    Year of Publishing2024
    Electronic addresshttps://pubs.acs.org/doi/full/10.1021/acs.analchem.2c03944
Number of the records: 1  

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