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Chemical Biopsy for GNMT as Noninvasive and Tumorigenesis-Relevant Diagnosis of Liver Cancer
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SYSNO ASEP 0568951 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chemical Biopsy for GNMT as Noninvasive and Tumorigenesis-Relevant Diagnosis of Liver Cancer Author(s) Oh, S. (KR)
Jo, S. (KR)
Kim, H. S. (KR)
Mai, V. (KR)
Endaya, B. (AU)
Neužil, Jiří (BTO-N) RID
Jung, K. H. (KR)
Hong, S. (KR)
Kim, J. (KR)
Park, S. (KR)Number of authors 10 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 95, č. 2 (2023), s. 1184-1192Number of pages 9 s. Language eng - English Country US - United States Keywords GLYCINE N-METHYLTRANSFERASE ; INFILTRATIVE HEPATOCELLULAR-CARCINOMA ; FOLATE-BINDING-PROTEIN Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Limited access Institutional support BTO-N - RVO:86652036 UT WOS 000923938900001 EID SCOPUS 85145985336 DOI 10.1021/acs.analchem.2c03944 Annotation Early diagnosis of hepatocellular carcinoma (HCC) is difficult, the patients burdened by life-long periodical examinations. Here, a new chemical biopsy approach was developed for noninvasive diagnosis of HCC using urine samples. Bioinformatic screening for tumor suppressors yielded glycine Nmethyltransferase (GNMT) as a biomarker with clinical relevance to HCC tumorigenesis. A liquid chromatography-mass spectrometry (LC-MS)-based chemical biopsy detecting nonradioactive 13C-sarcosine from 13C-glycine was designed to noninvasively assess liver GNMT activity extrahepatically. 13CSarcosine showed a strong correlation with GNMT in normal and cancerous liver cells. In an autochthonous animal model developing visible cancer nodules at 17 weeks, the urinary 13C-sarcosine chemical biopsy exhibited notable changes as early as 8 weeks, showing significant correlations with liver GNMT and molecular pathological changes. Our chemical biopsy approach should facilitate early and noninvasive diagnosis of HCC, with direct relevance to tumorigenesis, which can be straightforwardly applied to other diseases. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2024 Electronic address https://pubs.acs.org/doi/full/10.1021/acs.analchem.2c03944
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