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PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration
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SYSNO ASEP 0568881 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title PLCL/PCL dressings with platelet lysate and growth factors embedded in fibrin for chronic wound regeneration Author(s) Táborská, Johanka (UMCH-V)
Blanquer, Andreu (FGU-C) RID, ORCID
Brynda, Eduard (UMCH-V) RID
Filová, Elena (FGU-C) RID, ORCID
Stiborová, Lenka (UMCH-V)
Jenčová, V. (CZ)
Havlíčková, K. (CZ)
Riedelová, Zuzana (UMCH-V) ORCID
Riedel, Tomáš (UMCH-V) RID, ORCIDSource Title International Journal of Nanomedicine. - : Dove Medical Press
Roč. 18, 3 February (2023), s. 595-610Number of pages 16 s. Language eng - English Country NZ - New Zealand Keywords human platelet lysate ; diabetic ulcer ; fibrin coating Subject RIV FB - Endocrinology, Diabetology, Metabolism, Nutrition OECD category Endocrinology and metabolism (including diabetes, hormones) R&D Projects NV18-01-00332 GA MZd - Ministry of Health (MZ) LX22NPO5104 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823 UT WOS 000928064400001 EID SCOPUS 85147762343 DOI 10.2147/IJN.S393890 Annotation The formation of diabetic ulcers (DU) is a common complication for diabetic patients resulting in serious chronic wounds. There is therefore, an urgent need for complex treatment of this problem. This study examines a bioactive wound dressing of a biodegradable electrospun nanofibrous blend of poly(L-lactide-co-ϵ-caprolactone) and poly(ϵ-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained delivery of bioactive molecules. Electrospun PLCL/PCL nanofibers were coated with fibrin-based coating prepared by a controlled technique and enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF). The coating was characterized by scanning electron microscopy and fluorescent microscopy. Protein content and its release rate and the effect on human saphenous vein endothelial cells (HSVEC) were evaluated. The highest protein amount is achieved by the coating of PLCL/PCL with a fibrin mesh containing 20% v/v hPL (NF20). The fibrin coating serves as an excellent scaffold to accumulate bioactive molecules from hPL such as PDGF-BB, fibronectin (Fn), and α-2 antiplasmin. The NF20 coating shows both fast and a sustained release of the attached bioactive molecules (Fn, VEGF, FGF). The dressing significantly increases the viability of human saphenous vein endothelial cells (HSVECs) cultivated on a collagen-based wound model. The exogenous addition of FGF and VEGF during the coating procedure further increases the HSVECs viability. In addition, the presence of α-2 antiplasmin significantly stabilizes the fibrin mesh and prevents its cleavage by plasmin. The NF20 coating supplemented with FGF and VEGF provides a promising wound dressing for the complex treatment of DU. The incorporation of various bioactive molecules from hPL and growth factors has great potential to support the healing processes by providing appropriate stimuli in the chronic wound. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2024 Electronic address https://www.dovepress.com/plclpcl-dressings-with-platelet-lysate-and-growth-factors-embedded-in--peer-reviewed-fulltext-article-IJN
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