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Eda controls the size of the enamel knot during incisor development
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SYSNO ASEP 0568789 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Eda controls the size of the enamel knot during incisor development Author(s) Horáková, L. (CZ)
Dalecká, L. (CZ)
Zahradníček, Oldřich (UJF-V) ORCID, SAI
Lochovská, K. (CZ)
Lesot, Hervé (UZFG-Y)
Peterková, R. (CZ)
Trucker, A. S. (GB)
Hovořáková, M. (CZ)Number of authors 8 Article number 1033130 Source Title Frontiers in Physiology. - : Frontiers Research Foundation - ISSN 1664-042X
Roč. 13, JAN (2023)Number of pages 14 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords tabby mouse ; mouse incisor ; shh expression ; rudiment ; tooth development OECD category Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect) R&D Projects EF16_019/0000728 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 ; UJF-V - RVO:61389005 UT WOS 000921369500001 EID SCOPUS 85146907985 DOI 10.3389/fphys.2022.1033130 Annotation Ectodysplasin (Eda) plays important roles in both shaping the developing tooth and establishing the number of teeth within the tooth row. Sonic hedgehog (Shh) has been shown to act downstream of Eda and is involved in the initiation of tooth development. Eda-/- mice possess hypoplastic and hypomineralized incisors and show changes in tooth number in the molar region. In the present study we used 3D reconstruction combined with expression analysis, cell lineage tracing experiments, and western blot analysis in order to investigate the formation of the incisor germs in Eda-/- mice. We show that a lack of functional Eda protein during early stages of incisor tooth germ development had minimal impact on development of the early expression of Shh in the incisor, a region proposed to mark formation of a rudimental incisor placode and act as an initiating signalling centre. In contrast, deficiency of Eda protein had a later impact on expression of Shh in the primary enamel knot of the functional tooth. Eda-/- mice had a smaller region where Shh was expressed, and a reduced contribution from Shh descendant cells. The reduction in the enamel knot led to the formation of an abnormal enamel organ creating a hypoplastic functional incisor. Eda therefore appears to influence the spatial formation of the successional signalling centres during odontogenesis. Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2024 Electronic address https://doi.org/10.3389/fphys.2022.1033130
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