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Multiple roles of Pax6 in postnatal cornea development
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SYSNO ASEP 0568318 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Multiple roles of Pax6 in postnatal cornea development Author(s) Sunny, Sweetu Susan (UMG-J) ORCID
Láchová, Jitka (UMG-J)
Dupačová, Naoko (UMG-J)
Kozmik, Zbyněk (UMG-J) RIDSource Title Developmental Biology. - : Elsevier - ISSN 0012-1606
Roč. 491, Nov (2022), s. 1-12Number of pages 12 s. Language eng - English Country US - United States Keywords eye ; development ; cornea ; pax6 ; conditional knockout ; E-cadherin ; Keratins OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology R&D Projects LM2018126 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA21-27364S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UMG-J - RVO:68378050 UT WOS 000911781200001 EID SCOPUS 85137301689 DOI 10.1016/j.ydbio.2022.08.006 Annotation Mammalian corneal development is a multistep process, including formation of the corneal epithelium (CE), endothelium and stroma during embryogenesis, followed by postnatal stratification of the epithelial layers and continuous renewal of the epithelium to replace the outermost corneal cells. Here, we employed the Cre-loxP system to conditionally deplete Pax6 proteins in two domains of ocular cells, i.e., the ocular surface epithelium (cornea, limbus and conjunctiva) (OSE) or postnatal CE via K14-cre or Aldh3-cre, respectively. Earlier and broader inactivation of Pax6 in the OSE resulted in thickened OSE with CE and limbal cells adopting the conjunctival keratin expression pattern. More restricted depletion of Pax6 in postnatal CE resulted in an abnormal cornea marked by reduced epithelial thickness despite increased epithelial cell proliferation. Immunofluorescence studies revealed loss of intermediate filament Cytokeratin 12 and diffused expression of adherens junction components, together with reduced tight junction protein, Zonula occludens-1. Furthermore, the expression of Cytokeratin 14, a basal cell marker in apical layers, indicates impaired differentiation of CE cells. Collectively, our data demonstrate that Pax6 is essential for maintaining proper differentiation and strong intercellular adhesion in postnatal CE cells, whereas limbal Pax6 is required to prevent the outgrowth of conjunctival cells to the cornea. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2023 Electronic address https://www.sciencedirect.com/science/article/abs/pii/S0012160622001580?via%3Dihub
Number of the records: 1