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Full Assessment of Lung Mechanics UsingComputer-Controlled, Forced OscillationTechnique
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SYSNO ASEP 0568313 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve SCOPUS Title Full Assessment of Lung Mechanics UsingComputer-Controlled, Forced OscillationTechnique Author(s) de Guia, Roldan M. (UMG-J)
Žatečka, Václav (UMG-J)
Rozman, Jan (UMG-J)
Procházka, Jan (UMG-J) ORCID, RID
Sedláček, Radislav (UMG-J) RIDArticle number e488 Source Title Current Protocols - ISSN 2691-1299
Roč. 2, č. 7 (2022)Number of pages 26 s. Language eng - English Country US - United States Keywords endotracheal intubation ; FlexiVentFX ; forced oscillation technique ; lung function ; lung mechanics OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology R&D Projects LM2018126 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMG-J - RVO:68378050 EID SCOPUS 85134132715 DOI https://doi.org/10.1002/cpz1.488 Annotation The forced oscillation technique (FOT) is a powerful and accurate methodto quantify the mechanical properties of the airways and tissues of the res-piratory system. Here we provide a detailed protocol for the measurement ofmouse respiratory mechanical parameters. We present a procedure for mouseendotracheal intubation using a handcrafted intubation platform and confir-mation module. The FlexiVentFXTMsystem (Scireq Inc.) is utilized for thethorough assessment of lung function with the FlexiWareTMsoftware serv-ing as a unit for the planning, experimentation, and analysis. The protocol hasbeen standardized and adapted for use by our center for lung-function pheno-typing of mouse models generated for the International Mouse PhenotypingConsortium (IMPC). The simplified steps, technical considerations, and inte-grated hardware-software demonstration make this protocol adaptable and im-plementable for researchers interested in using FOT for lung-function evaluation Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2023 Electronic address https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.488
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