Hard tissues on a chip – development of a platform for generating 3D bone and cartilage cultures

0566670 - ÚIACH 2023 CZ eng A - Abstract
Killinger, Michael - Szotkovská, Tereza - Václavek, Tomáš - Buchtová, Marcela - Klepárník, Karel
Hard tissues on a chip – development of a platform for generating 3D bone and cartilage cultures.
Czech Chemical Society Symposium Series. "Amerika" 21st, č. 20 (2022), s. 21-22. ISSN 2336-7202.
[Mezioborové setkání mladých biologů, biochemiků a chemiků /21./. 16.05.2022-19.05.2022, Milovy]
R&D Projects: GA ČR(CZ) GA20-00726S
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : 3D Bone In Vitro Models * microfluidics
OECD category: Analytical chemistry

In this study, we developed a polydimethylsiloxane (PDMS) microfluidic platform suitable for 3D bone and cartilaginous cells cultivation. The proper fabrication and preparation led to the development of homogenous 3D cell cultures of MC3T3-E1 osteoblast cells. Moreover, the system was used to study primary chondrocyte differentiation while using WNT inhibitors. These protocols were compared to standard cultivation methods such as hanging drops to reflect the advantages of microfluidic platforms.
Permanent Link: https://hdl.handle.net/11104/0337982