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Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions
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SYSNO ASEP 0566604 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions Author(s) Abhinav, Abhinav (UFCH-W)
Jurkiewicz, Piotr (UFCH-W) RID, ORCID
Hof, Martin (UFCH-W) RID, ORCID
Allolio, C. (CZ)
Sýkora, Jan (UFCH-W) RIDArticle number 1894 Source Title Biomolecules. - : MDPI
Roč. 12, č. 12 (2022)Number of pages 13 s. Language eng - English Country CH - Switzerland Keywords phospholipid bilayer ; calcium ; proton ; anionic lipids ; headgroup organization ; molecular dynamics ; time dependent fluorescence shift ; Laurdan ; lipid hydration ; headgroup mobility Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GA22-25953S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UFCH-W - RVO:61388955 UT WOS 000902282700001 EID SCOPUS 85144710558 DOI 10.3390/biom12121894 Annotation Biomembranes, important building blocks of living organisms, are often exposed to large local fluctuations of pH and ionic strength. To capture changes in the membrane organization under such harsh conditions, we investigated the mobility and hydration of zwitterionic and anionic lipid bilayers upon elevated H3O+ and Ca2+ content by the time-dependent fluorescence shift (TDFS) technique. While the zwitterionic bilayers remain inert to lower pH and increased calcium concentrations, anionic membranes are responsive. Specifically, both bilayers enriched in phosphatidylserine (PS) and phosphatidylglycerol (PG) become dehydrated and rigidified at pH 4.0 compared to at pH 7.0. However, their reaction to the gradual Ca2+ increase in the acidic environment differs. While the PG bilayers exhibit strong rehydration and mild loosening of the carbonyl region, restoring membrane properties to those observed at pH 7.0, the PS bilayers remain dehydrated with minor bilayer stiffening. Molecular dynamics (MD) simulations support the strong binding of H3O+ to both PS and PG. Compared to PS, PG exhibits a weaker binding of Ca2+ also at a low pH. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2023 Electronic address https://hdl.handle.net/11104/0337920
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