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An autoactive NB-LRR gene causes Rht13 dwarfism in wheat
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SYSNO ASEP 0566545 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title An autoactive NB-LRR gene causes Rht13 dwarfism in wheat Author(s) Borrill, P. (GB)
Mago, R. (AU)
Xu, T. (CN)
Ford, B. A. (AU)
Williams, S. J. (AU)
Derkx, A. (AU)
Bovill, W. D. (AU)
Hyles, J. (GB)
Bhatt, D. (AU)
Xia, X. (SA)
MacMillan, C. (AU)
White, R. (US)
Buss, W. (AU)
Molnár, István (UEB-Q) ORCID
Walkowiak, S. (CA)
Olsen, O.A. (NO)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Pozniak, C. (CA)
Spielmeyer, W. (AU)Number of authors 19 Article number e2209875119 Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 119, č. 48 (2022)Number of pages 9 s. Language eng - English Country US - United States Keywords autoactive NB-LRR ; reduced-height (Rht) gene ; semidwarfing gene ; Triticum aestivum L. (wheat) OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 001043103000005 EID SCOPUS 85142939678 DOI https://doi.org/10.1073/pnas.2209875119 Annotation Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1073/pnas.2209875119
Number of the records: 1