Can classical surface plasmon resonance advance via the coupling to other analytical approaches?

0566272 - FZÚ 2023 RIV CH eng J - Journal Article
Geilfuss, D. - Boukherroub, R. - Dostálek, Jakub - Knoll, W. - Masson, J.F. - Baeumner, A.J. - Szunerits, S.
Can classical surface plasmon resonance advance via the coupling to other analytical approaches?
Frontiers in Analytical Science. Roč. 2, Dec. (2022), č. článku 1091869. E-ISSN 2673-9283
R&D Projects: GA ČR(CZ) GC22-30456J
Institutional support: RVO:68378271
Keywords : analytical science * surface plasmon resonace (SPR) * mass spectroscopic * electrochemistry * lateral flow assay (LFA)
OECD category: Biophysics
Method of publishing: Open access

For nearly 40 years, surface plasmon resonance (SPR) analysis has been used to better understand the binding interaction strength between surface immobilized bioreceptors and the analytes of interest. The advantage of surface plasmon resonance, over other affinity sensing approaches such as Western blots and ELISAs approaches, resides in its possibility to reveal binding kinetics in a label-free manner. The concept of surface plasmon resonance has in addition been widely employed for the development of biosensors capitalizing on its direct assay format, short response times, simple sample treatments along with multiplexed sensing possibilities. To this must be added the possibility to reach high sensitivity due to the capability of surface plasmon resonance to detect very small changes in refractive index at the sensing interfaces in particular for analytes of larger size such as cells (e.g., bacteria), proteins, peptides and oligonucleotides.
Permanent Link: https://hdl.handle.net/11104/0339377