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Growth suppression of bacteria by biofilm deterioration using silver nanoparticles with magnetic doping
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SYSNO ASEP 0565981 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Growth suppression of bacteria by biofilm deterioration using silver nanoparticles with magnetic doping Author(s) Torres-Mendieta, R. (CZ)
Nguyen, N. H. A. (CZ)
Guadagnini, A. (IT)
Semerád, Jaroslav (MBU-M) ORCID, RID
Lukowiec, D. (PL)
Parma, P. (CZ)
Yang, J. (IT)
Agnoli, S. (IT)
Ševců, A. (CZ)
Cajthaml, Tomáš (MBU-M) RID, ORCID
Cernik, M. (CZ)
Amendola, V. (IT)Source Title Nanoscale. - : Royal Society of Chemistry - ISSN 2040-3364
Roč. 14, č. 48 (2022), s. 18143-18156Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords iron-oxide nanoparticles ; laser-ablation ; alloy nanoparticles ; icp-ms ; cobalt ; antibacterial ; stressmetalsliquid ; metal ; sliquid Subject RIV EE - Microbiology, Virology OECD category Microbiology Research Infrastructure NanoEnviCz II - 90124 - Ústav fyzikální chemie J. Heyrovského AV ČR, v. v. i. Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000892544200001 EID SCOPUS 85144023669 DOI 10.1039/d2nr03902h Annotation Decades of antibiotic use and misuse have generated selective pressure toward the rise of antibiotic-resistant bacteria, which now contaminate our environment and pose a major threat to humanity. According to the evolutionary ´Red queen theory´, developing new antimicrobial technologies is both urgent and mandatory. While new antibiotics and antibacterial technologies have been developed, most fail to penetrate the biofilm that protects bacteria against external antimicrobial attacks. Hence, new antimicrobial formulations should combine toxicity for bacteria, biofilm permeation ability, biofilm deterioration capability, and tolerability by the organism without renouncing compatibility with a sustainable, low-cost, and scalable production route as well as an acceptable ecological impact after the ineluctable release of the antibacterial compound in the environment. Here, we report on the use of silver nanoparticles (NPs) doped with magnetic elements (Co and Fe) that allow standard silver antibacterial agents to perforate bacterial biofilms through magnetophoretic migration upon the application of an external magnetic field. The method has been proved to be effective in opening micrometric channels and reducing the thicknesses of models of biofilms containing bacteria such as Enterococcus faecalis, Enterobacter cloacae, and Bacillus subtilis. Besides, the NPs increase the membrane lipid peroxidation biomarkers through the formation of reactive oxygen species in E. faecalis, E. cloacae, B. subtilis, and Pseudomonas putida colonies. The NPs are produced using a one-step, scalable, and environmentally low-cost procedure based on laser ablation in a liquid, allowing easy transfer to real-world applications. The antibacterial effectiveness of these magnetic silver NPs may be further optimized by engineering the external magnetic fields and surface conjugation with specific functionalities for biofilm disruption or bactericidal effectiveness. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://pubs.rsc.org/en/content/articlelanding/2022/NR/D2NR03902H
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