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H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin
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SYSNO ASEP 0563716 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin Author(s) de la Iglesia, O. (ES)
Jauregi, P. (ES)
Jodar, M. (ES)
Barrachina, F. (ES)
Děd, Lukáš (BTO-N) RID
Mallofre, C. (ES)
Rodriguez-Carunchio, L. (ES)
Manuel Corral, J. (ES)
Lluis Ballesca, J. (ES)
Komrsková, Kateřina (BTO-N) ORCID
Castillo, J. (ES)
Oliva, R. (ES)Number of authors 12 Article number 12398 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 20 (2022)Number of pages 18 s. Language eng - English Country CH - Switzerland Keywords butyrylation ; acetylation ; spermatogenesis ; sperm ; sperm chromatin ; epigenetic regulation Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GJ20-17403Y GA ČR - Czech Science Foundation (CSF) GC20-20217J GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 000875419200001 DOI 10.3390/ijms232012398 Annotation Male germ cells experience a drastic chromatin remodeling through the nucleo-histone to nucleo-protamine (NH-NP) transition necessary for proper sperm functionality. Post-translational modifications (PTMs) of H4 Lys5, such as acetylation (H4K5ac), play a crucial role in epigenetic control of nucleosome disassembly facilitating protamine incorporation into paternal DNA. It has been shown that butyrylation on the same residue (H4K5bu) participates in temporal regulation of NH-NP transition in mice, delaying the bromodomain testis specific protein (BRDT)-dependent nucleosome disassembly and potentially marking retained nucleosomes. However, no information was available so far on this modification in human sperm. Here, we report a dual behavior of H4K5bu and H4K5ac in human normal spermatogenesis, suggesting a specific role of H4K5bu during spermatid elongation, coexisting with H4K5ac although with different starting points. This pattern is stable under different testicular pathologies, suggesting a highly conserved function of these modifications. Despite a drastic decrease of both PTMs in condensed spermatids, they are retained in ejaculated sperm, with 30% of non-colocalizing nucleosome clusters, which could reflect differential paternal genome retention. Whereas no apparent effect of these PTMs was observed associated with sperm quality, their presence in mature sperm could entail a potential role in the zygote. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2023 Electronic address https://www.mdpi.com/1422-0067/23/20/12398
Number of the records: 1