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Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice
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SYSNO ASEP 0562817 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice Author(s) Votta, C. (IT)
Fiorilli, V. (IT)
Haider, I. (SA)
Wang, J.Y. (CN)
Balestrini, R. (IT)
Petřík, Ivan (UEB-Q) ORCID
Tarkowská, Danuše (UEB-Q) RID, ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Serikbayeva, A. (SA)
Bonfante, P. (IT)
Al-Babili, S. (SA)
Lanfranco, L. (IT)Number of authors 12 Source Title Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 111, č. 6 (2022), s. 1688-1700Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords apocarotenoids ; arbuscular mycorrhizal symbiosis ; gr24 ; in situ hybridization ; Oryza sativa ; OsPT11 ; strigolactones ; zaxinone ; zaxinone synthase OECD category Biochemical research methods R&D Projects EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000841166000001 EID SCOPUS 85135900324 DOI 10.1111/tpj.15917 Annotation The Oryza sativa (rice) carotenoid cleavage dioxygenase OsZAS was described to produce zaxinone, a plant growth-promoting apocarotenoid. A zas mutant line showed reduced arbuscular mycorrhizal (AM) colonization, but the mechanisms underlying this behavior are unknown. Here, we investigated how OsZAS and exogenous zaxinone treatment regulate mycorrhization. Micromolar exogenous supply of zaxinone rescued root growth but not the mycorrhizal defects of the zas mutant, and even reduced mycorrhization in wild-type and zas genotypes. The zas line did not display the increase in the level of strigolactones (SLs) that was observed in wild-type plants at 7 days post-inoculation with AM fungus. Moreover, exogenous treatment with the synthetic SL analog GR24 rescued the zas mutant mycorrhizal phenotype, indicating that the lower AM colonization rate of zas is caused by a deficiency in SLs at the early stages of the interaction, and indicating that during this phase OsZAS activity is required to induce SL production, possibly mediated by the Dwarf14-Like (D14L) signaling pathway. OsZAS is expressed in arbuscule-containing cells, and OsPT11prom::OsZAS transgenic lines, where OsZAS expression is driven by the OsPT11 promoter active in arbusculated cells, exhibit increased mycorrhization compared with the wild type. Overall, our results show that the genetic manipulation of OsZAS activity in planta leads to a different effect on AM symbiosis from that of exogenous zaxinone treatment, and demonstrate that OsZAS influences the extent of AM colonization, acting as a component of a regulatory network that involves SLs. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.1111/tpj.15917
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