Number of the records: 1
Diamond Surfaces with Clickable Antifouling Polymer Coating for Microarray-Based Biosensing
- 1.
SYSNO ASEP 0562670 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Diamond Surfaces with Clickable Antifouling Polymer Coating for Microarray-Based Biosensing Author(s) Kumar, R. (DE)
Yang, B. (DE)
Bartoň, Jan (UOCHB-X) RID, ORCID
Štejfová, Miroslava (UOCHB-X) ORCID
Schaefer, A. (DE)
König, M. (DE)
Knittel, P. (DE)
Cígler, Petr (UOCHB-X) RID, ORCID
Hirtz, M. (DE)Article number 2201453 Source Title Advanced Materials Interfaces. - : Wiley - ISSN 2196-7350
Roč. 9, č. 33 (2022)Number of pages 10 s. Language eng - English Country DE - Germany Keywords antifouling polymer coating ; copper catalyzed alkyne-azide cycloaddition ; diamond surfaces ; fluorescence imaging ; microchannel cantilever spotting ; selective protein binding OECD category Materials engineering R&D Projects EF16_026/0008382 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UOCHB-X - RVO:61388963 UT WOS 000865567900001 EID SCOPUS 85139453225 DOI https://doi.org/10.1002/admi.202201453 Annotation Diamond enables the construction of various (bio)sensors, including those with quantum-based detection. However, bare diamond interfaces are susceptible to unspecific adhesion of proteins and other macromolecules from biological media or complex samples. This impairs selectivity in biosensing, leads to low signal-to-noise ratio in fluorescence-based applications, and introduces the need for blocking steps in incubation protocols. Here, a stable, protein-repellent, and clickable reactive polymer coating is introduced, abolishing unspecific protein adhesion while concurrently enabling covalent immobilization of functional compounds as recognition elements. The polymer coating has two segments, an antifouling poly(N-(2-hydroxypropyl) methacrylamide) and an alkyne-terminated poly(propargyl methacrylamide) providing the click functionality. The antifouling properties and click-reactivity of the polymers are demonstrated by selective protein binding assays on micropatterns written by microchannel cantilever spotting (mu CS). The assays demonstrated the successful functionalization of both diamond and glass surfaces and the excellent antifouling properties of the polymer coating. The coating procedure is compatible with oxidized diamond surfaces thus well-suitable for diamond-based quantum technology. The results can directly impact applications of diamond materials in optically detected quantum sensing or fluorescence sensing in general. The polymer functionalization can also be used for any case where highly specific interaction with low fouling is desired. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2023 Electronic address https://doi.org/10.1002/admi.202201453
Number of the records: 1