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SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2, and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet-plasma membrane tethering

  1. 1.
    0562423 - ÚEB 2023 RIV US eng J - Journal Article
    Krawczyk, H. E. - Sun, S. - Doner, N. M. - Yan, Q. - Lim, M. S. S. - Scholz, P. - Niemeyer, P. W. - Schmitt, K. - Valerius, K. - Pleskot, Roman - Hillmer, S. - Braus, G. H. - Wiermer, M. - Mullen, R. T. - Ischebeck, T.
    SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2, and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet-plasma membrane tethering.
    Plant Cell. Roč. 34, č. 6 (2022), s. 2424-2448. ISSN 1040-4651. E-ISSN 1532-298X
    Institutional support: RVO:61389030
    Keywords : ACID BETA-OXIDATION * COILED-COIL * TRIACYLGLYCEROL LIPASE
    OECD category: Cell biology
    Impact factor: 12.0, year: 2022
    Method of publishing: Open access
    https://doi.org/10.1093/plcell/koac095

    Membrane contact sites (MCSs) are interorganellar connections that allow for the direct exchange of molecules, such as lipids or Ca2+ between organelles, but can also serve to tether organelles at specific locations within cells. Here, we identified and characterized three proteins of Arabidopsis thaliana that form a lipid droplet (LD)-plasma membrane (PM) tethering complex in plant cells, namely LD-localized SEED LD PROTEIN (SLDP) 1 and SLDP2 and PM-localized LD-PLASMA MEMBRANE ADAPTOR (LIPA). Using proteomics and different protein-protein interaction assays, we show that both SLDPs associate with LIPA. Disruption of either SLDP1 and SLDP2 expression, or that of LIPA, leads to an aberrant clustering of LDs in Arabidopsis seedlings. Ectopic co-expression of one of the SLDPs with LIPA is sufficient to reconstitute LD-PM tethering in Nicotiana tabacum pollen tubes, a cell type characterized by dynamically moving LDs in the cytosolic streaming. Furthermore, confocal laser scanning microscopy revealed both SLDP2.1 and LIPA to be enriched at LD-PM contact sites in seedlings. These and other results suggest that SLDP and LIPA interact to form a tethering complex that anchors a subset of LDs to the PM during post-germinative seedling growth in Arabidopsis.
    Permanent Link: https://hdl.handle.net/11104/0334739

     
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