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New insights into ribosomal DNA variation in apomictic and sexual Taraxacum (Asteraceae)
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SYSNO ASEP 0562420 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title New insights into ribosomal DNA variation in apomictic and sexual Taraxacum (Asteraceae) Author(s) Macháčková, I. (CZ)
Majeský, L. (CZ)
Hroneš, M. (CZ)
Bílková, Lucie (UEB-Q)
Hřibová, Eva (UEB-Q) RID, ORCID
Vašut, R.J. (CZ)Number of authors 6 Source Title Botanical Journal of the Linnean Society. - : Oxford University Press - ISSN 0024-4074
Roč. 199, č. 4 (2022), s. 790-815Number of pages 26 s. Language eng - English Country GB - United Kingdom Keywords apomixis ; concerted evolution ; fish ; GC content ; genome size ; karyotype ; metaphase chromosomes ; rDNA OECD category Genetics and heredity (medical genetics to be 3) R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000756758600001 EID SCOPUS 85136228501 DOI 10.1093/botlinnean/boab094 Annotation Apomictic genera have a complex evolutionary history, including reticulate hybridization, polyploidization and variation in reproduction modes. Restrictions of functional meiosis in polyploid apomictic taxa considerably hamper gene conversion, leaving footprints of past hybridization events in their genomes, which are masked by the homogenization of tandemly organized arrays of nuclear ribosomal DNA in sexual species. Dandelions (Taraxacum) have a highly complex reticulate evolutionary history. Detailed knowledge remains hazy, earlier investigations of rDNA sequences have uncovered the complex pattern derived from evolution but without direct evidence for any particular processes. We investigated the position and number of 45S and 5S rDNA loci in 38 Taraxacum taxa (covering different reproduction modes, geographical regions and putative phylogenetic groups) using fluorescent in situ hybridization (FISH) and measured genome size and GC content. The ITS1-5.8S-ITS2 regions of four sexual and five apomictic taxa were sequenced to investigate inter- and intra-individual variation. Most species considerably differ in the chromosome positions of loci and karyotype patterns, but mostly share the same number of studied loci (45S:5S) in a 1:2 ratio per haploid genome (x = 8), with six exceptions (up to a 4:2 ratio). Genome size (2C) varies sixfold and with GC content partly distinguishes major evolutionary groups. Sexual taxa show limited variation in sequenced regions (with two to eight variants), but apomictic taxa vary significantly (with 20-36 variants). Extensive reticulate evolution in Taraxacum and subsequent phenomena such as genome repatterning and non-effective concerted evolution are probably the cause of the dynamic nature of Taraxacum karyotypes and the large variation in genome size and rDNA sequences. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.1093/botlinnean/boab094
Number of the records: 1