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Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis
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SYSNO ASEP 0562417 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis Author(s) Antoniadi, I. (SE)
Skalický, Vladimír (UEB-Q) ORCID
Sun, G. (CN)
Ma, W. (CN)
Galbraith, D. W. (US)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Ljung, K. (SE)Number of authors 7 Source Title Cytometry. Part A. - : Wiley - ISSN 1552-4922
Roč. 101, č. 9 (2022), s. 725-736Number of pages 12 s. Language eng - English Country US - United States Keywords autofluorescence ; best practices ; plant flow cytometry and sorting ; protoplasts ; viability and integrity OECD category Biochemistry and molecular biology R&D Projects GJ17-21581Y GA ČR - Czech Science Foundation (CSF) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000653233000001 EID SCOPUS 85106344118 DOI 10.1002/cyto.a.24461 Annotation Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.1002/cyto.a.24461
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