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Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels
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SYSNO ASEP 0562009 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels Author(s) Hlaváček, Antonín (UIACH-O) ORCID
Křivánková, Jana (UIACH-O) RID, ORCID
Brožková, Hana (UIACH-O)
Weisová, Julie (UIACH-O)
Pizúrová, Naděžda (UFM-A) RID, ORCID
Foret, František (UIACH-O) RID, ORCIDNumber of authors 6 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 94, č. 41 (2022), s. 14340-14348Number of pages 9 s. Publication form Print - P Language eng - English Country US - United States Keywords absolute quantification ; counting ; photon-upconversion ; fluorescence ; nanoparticles Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Subject RIV - cooperation Institute of Physics of Materials - Analytical Chemistry, Separation R&D Projects GA21-03156S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 ; UFM-A - RVO:68081723 UT WOS 000870033800001 EID SCOPUS 85139477673 DOI 10.1021/acs.analchem.2c02989 Annotation The presented method is suitable for estimating the number concentration of nanoparticles and their bioconjugates. The method benefits from well-defined immobilization of nanomaterials in anisotropically collapsed agarose gel. Once immobilized, the nanoparticles are imaged by a microscope and counted. The number of counted nanoparticles is then used for estimating the number concentration. The accuracy and precision of the method were characterized by the samples of photon-upconversion nanoparticles. By analyzing the brightness of the emission from single diffraction-limited spots, it was possible to analyze also the content of UCNP clusters. The detection of extremely weak emitters was proved. The method supports several microscope imaging modalities such as photon-upconversion, fluorescence, dark-field, and bright-field microscopy. The applicability of these modalities is demonstrated by imaging UCNPs, dye-doped fluorescent silica nanoparticles, CdSe/ZnS quantum dots, and submicron silica particles. Thus, the method is not limited to a particular nanomaterial or imaging modality type. The method was developed for aqueous dispersions of nanoparticles and utilized the agarose gel to provide a well-defined immobilization of nanomaterials. However, the gels are not limited to only agarose or aqueous dispersions. Therefore, the method can be likely extended to other types of solvents and gel matrices. Interestingly, agarose contains only light elements such as carbon, oxygen, hydrogen, and a small amount of sulfur. Therefore, the method is potentially compatible with TEM, which can provide an even more detailed analysis. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2023 Electronic address https://hdl.handle.net/11104/0334437
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