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Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect
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SYSNO ASEP 0560260 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect Author(s) Jasenská, D. (CZ)
Kašpárková, V. (CZ)
Vašíček, Ondřej (BFU-R) ORCID, RID
Münster, L. (CZ)
Minařík, A. (CZ)
Kacerova, S. (CZ)
Korabkova, E. (CZ)
Urbánková, L. (CZ)
Vícha, J. (CZ)
Capáková, Z. (CZ)
Falleta, E. (IT)
Della Pina, C. (IT)
Lehocký, M. (CZ)
Skopalová, K. (CZ)
Humpolíček, P. (CZ)Number of authors 15 Source Title Biomacromolecules. - : American Chemical Society - ISSN 1525-7797
Roč. 23, č. 8 (2022), s. 3359-3370Number of pages 12 s. Publication form Print - P Language eng - English Country US - United States Keywords ANTIOXIDANT ACTIVITY ; NANOPARTICLES ; ANILINE Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000835322900001 EID SCOPUS 85135599232 DOI 10.1021/acs.biomac.2c00371 Annotation A green, nature-friendly synthesis of polyaniline colloidal particles based on enzyme-assisted oxidation of aniline with horseradish peroxidase and chitosan or poly(vinyl alcohol) as steric stabilizers was successfully employed. Physicochemical characterization revealed formation of particles containing the polyaniline emeraldine salt and demonstrated only a minor effect of polymer stabilizers on particle morphology. All tested colloidal particles showed in vitro antioxidation activity determined via scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. In vitro, they were able to reduce oxidative stress and inhibit the production of reactive oxygen species by neutrophils and inflammatory cytokines by macrophages. The anti-inflammatory effect observed was related to their antioxidant activity, especially in the case of neutrophils. The particles can thus be especially advantageous as active components of biomaterials modulating the early stages of inflammation. In addition to the immunomodulatory effect, the presence of intrinsically conducting polyaniline can impart cell-instructive properties to the particles. The approach to particle synthesis that we employed-an original one using environmentally friendly and biocompatible horseradish peroxidase-represents a smart way of preparing conducting particles with unique properties, which can be further modified by the stabilizers used. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2023 Electronic address https://pubs.acs.org/doi/10.1021/acs.biomac.2c00371
Number of the records: 1