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Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
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SYSNO ASEP 0559856 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition. Author(s) Kronusová, O. (CZ)
Kaštánek, P. (CZ)
Koyun, G. (CZ)
Kaštánek, František (UCHP-M) RID, SAI, ORCID
Brányik, T. (CZ)Number of authors 5 Article number 1473 Source Title Microorganisms. - : MDPI
Roč. 10, č. 7 (2022)Number of pages 14 s. Language eng - English Country CH - Switzerland Keywords dictyosphaerium chlorelloides ; extracellular polysaccharides ; photobioreactor OECD category Chemical process engineering Method of publishing Open access Institutional support UCHP-M - RVO:67985858 UT WOS 000833658200001 EID SCOPUS 85136375827 DOI 10.3390/microorganisms10071473 Annotation The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS). Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL. Multiple limitations in nutrient supply were proven to be an effective method for EPS overproduction. Salinity stress was also applied to the culture, but no significant increase in EPS production was observed. The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate. Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation. Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L. A new and efficient methodology was developed for separating cells from the EPS-containing culture broth. Due to the strong attachment between cells and EPS, high-pressure homogenization was carried out before a centrifugation process. The EPS in the supernatant was subsequently purified using ultrafiltration. The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2023 Electronic address https://www.mdpi.com/2076-2607/10/7/1473/htm
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