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Nanodiamond-Quantum Sensors Reveal Temperature Variation Associated to Hippocampal Neurons Firing
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SYSNO ASEP 0559552 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nanodiamond-Quantum Sensors Reveal Temperature Variation Associated to Hippocampal Neurons Firing Author(s) Petrini, G. (IT)
Tomagra, G. (IT)
Bernardi, E. (IT)
Moreva, E. (IT)
Traina, P. (IT)
Marcantoni, A. (IT)
Picollo, F. (IT)
Kvaková, Klaudia (UOCHB-X) ORCID
Cígler, Petr (UOCHB-X) RID, ORCID
Degiovanni, I. P. (IT)
Carabelli, V. (IT)
Genovese, M. (IT)Article number 2202014 Source Title Advanced Science. - : Wiley
Roč. 9, č. 28 (2022)Number of pages 11 s. Language eng - English Country US - United States Keywords intracellular nanoscale sensing ; nanodiamonds ; nitrogen-vacancy (NV) centers ; ODMR OECD category Nano-materials (production and properties) R&D Projects EF16_026/0008382 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UOCHB-X - RVO:61388963 UT WOS 000830459300001 EID SCOPUS 85134571799 DOI 10.1002/advs.202202014 Annotation Temperature is one of the most relevant parameters for the regulation of intracellular processes. Measuring localized subcellular temperature gradients is fundamental for a deeper understanding of cell function, such as the genesis of action potentials, and cell metabolism. Notwithstanding several proposed techniques, at the moment detection of temperature fluctuations at the subcellular level still represents an ongoing challenge. Here, for the first time, temperature variations (1 °C) associated with potentiation and inhibition of neuronal firing is detected, by exploiting a nanoscale thermometer based on optically detected magnetic resonance in nanodiamonds. The results demonstrate that nitrogen-vacancy centers in nanodiamonds provide a tool for assessing various levels of neuronal spiking activity, since they are suitable for monitoring different temperature variations, respectively, associated with the spontaneous firing of hippocampal neurons, the disinhibition of GABAergic transmission and the silencing of the network. Conjugated with the high sensitivity of this technique (in perspective sensitive to < 0.1 °C variations), nanodiamonds pave the way to a systematic study of the generation of localized temperature gradients under physiological and pathological conditions. Furthermore, they prompt further studies explaining in detail the physiological mechanism originating this effect. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2023 Electronic address https://doi.org/10.1002/advs.202202014
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