Organosulfur/selenium-based Highly Fluorogenic Molecular Probes for Live-Cell Nucleolus Imaging

0556556 - ÚOCHB 2023 RIV DE eng J - Journal Article
Mondal, I. C. - Galkin, Maksym - Sharma, S. - Murugan, N. A. - Yushchenko, Dmytro A. - Girdhar, K. - Karmakar, A. - Mondal, P. - Gaur, Pankaj - Ghosh, S.
Organosulfur/selenium-based Highly Fluorogenic Molecular Probes for Live-Cell Nucleolus Imaging.
Chemistry-An Asian Journal. Roč. 17, č. 7 (2022), č. článku e202101281. ISSN 1861-4728. E-ISSN 1861-471X
Institutional support: RVO:61388963
Keywords : conformational dynamics * fluorescent probe * nucleic acid * nucleolus imaging * organochalcogens
OECD category: Biochemistry and molecular biology
Impact factor: 4.1, year: 2022
Method of publishing: Limited access
https://doi.org/10.1002/asia.202101281

We present fluorogenic cationic organo chalcogens that are highly selective to RNA. We have demonstrated that the conformational dynamics and subsequently the optical properties of these dyes can be controlled to facilitate efficient bioimaging. We report the application of organoselenium and organosulfur-based cell-permeable red-emissive probes bearing a favorable cyclic sidearm for selective and high contrast imaging of cell nucleoli. The probes exhibit high quantum yield upon interacting with RNA in an aqueous solution. An in-depth multiscale simulation study reveals that the prominent rotational freezing of the electron-donating sidearm of the probes in the microenvironment of RNA helps in attaining more planar conformation when compared to DNA. It exerts a greater extent of intramolecular charge transfer and hence leads to enhanced fluorescence emission. A systematic structure-interaction relationship study highlighted the impact of heavy-chalcogens toward the improved emissive properties of the probes.
Permanent Link: http://hdl.handle.net/11104/0331015