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IMG Electron Microscopy Core Facility
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SYSNO ASEP 0555851 Document Type O - Others R&D Document Type Others Title IMG Electron Microscopy Core Facility Author(s) Filimonenko, Vlada (UMG-J) RID, ORCID
Pinkas, Dominik (UMG-J) ORCID
Vlčák, Erik (UMG-J)
Raabová, Helena (UMG-J)
Hozák, Pavel (UMG-J) RID, ORCIDYear of issue 2021 Language eng - English Country CZ - Czech Republic Keywords Transmission lectron microscopy ; sample preparation ; Czech-BioImaging ; Euro-BioImgaging Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) TE01020118 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Research Infrastructure Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Institutional support UMG-J - RVO:68378050 Annotation The Electron Microscopy Core Facility provides expertise and cutting-edge equipment for a broad range of biological sample preparation and ultrastructural imaging techniques. The core facility deals with various biological samples: human and animal cell cultures, plant and animal tissues, worms, microorganisms, lipid micelles, isolated DNA. The sample preparation techniques include routine chemical fixation and resin embedding, negative staining, cryofixation using plunge-freezing or high-pressure freezing, freeze-substitution, cryosectioning, freeze fracture replica labeling, pre- and post-embedding immunolabeling, the list being lately extended by cryoCLEM using specialized microscope.
High-pressure freezer, two automatic freeze-substitution machines, cryo-ultramicrotomes, automated plunge-freezer, freeze-fracture replica making device, cryoCLEM microscope, as well as additional wet lab equipment are available. Transmission electron microscopes (TEM) installed in the end of 2019 include a standard 120 kV instrument for routine observation and an advanced 200 kV S/TEM providing the possibility of high-resolution TEM, 3D electron tomography, cryoelectron microscopy and EDS elemental analysis.
The team has a long expertise in the development and optimization of sample preparation techniques, including fruitful collaborations with companies providing equipment for electron microscopy.
The Electron Microscopy Core Facility is part of the IMG Czech-BioImaging node and Prague Euro-BioImaging node. We provide open access to our technologies and expertise and are ready to welcome users from all fields.Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2022
Number of the records: 1