Number of the records: 1
The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation
- 1.
SYSNO ASEP 0549779 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation Author(s) Moravcová, S. (CZ)
Filipovská, E. (CZ)
Spišská, V. (CZ)
Svobodová, Irena (FGU-C) RID, ORCID
Novotný, J. (CZ)
Bendová, Z. (CZ)Article number 1105 Source Title Life. - : MDPI
Roč. 11, č. 10 (2021)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords STAT3 ; arylalkylamine-N-acetyltransferase ; pineal gland ; circadian rhythms ; lipopolysaccharide ; rat Subject RIV ED - Physiology OECD category Physiology (including cytology) R&D Projects EF16_025/0007444 GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000716757000001 EID SCOPUS 85118144507 DOI 10.3390/life11101105 Annotation In rodents, the melatonin production by the pineal gland is controlled through adrenergic signaling from the suprachiasmatic nuclei and regulation of the principal enzyme in its synthesis, arylalkylamine-N-acetyltransferase (AANAT). In the present study, we identified increased isoprenaline-induced aa-nat expression and nocturnal AANAT activity in the pineal glands in response to the silencing of the signal transducer and activator of transcription 3 (STAT3) with siRNA or STAT3 inhibitors WP1066 and AZD1480. This AANAT activity enhancement in vivo did not interfere with light-induced AANAT suppression. Systemic or in vitro lipopolysaccharide (LPS) administration markedly increased Stat3 expression and STAT3 phosphorylation, but it did not significantly affect AANAT expression or activity. Simultaneous LPS administration and Stat3 silencing enhanced the aa-nat transcription and AANAT activity to a similar extent as Stat3 inhibition without LPS co-administration. Furthermore, we describe the circadian rhythmicity in Stat3 expression and the phosphorylated form of STAT3 protein in the rat pineal gland. Our data suggest that the higher nocturnal endogenous level of STAT3 in the pineal gland decelerates or hampers the process of NA-induced AANAT activation or affects the AANAT enzyme stability. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://www.mdpi.com/2075-1729/11/10/1105
Number of the records: 1