Number of the records: 1
Strongyloides genotyping: a review of methods and application in public health and population genetics
- 1.
SYSNO ASEP 0548740 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Strongyloides genotyping: a review of methods and application in public health and population genetics Author(s) Bradbury, R. S. (AU)
Pafčo, Barbora (UBO-W) RID, ORCID, SAI
Nosková, E. (CZ)
Hasegawa, H. (JP)Number of authors 4 Source Title International Journal for Parasitology. - : Elsevier - ISSN 0020-7519
Roč. 51, 13-14 (2021), s. 1153-1166Number of pages 14 s. Language eng - English Country AU - Australia Keywords Epidemiology ; Genome ; Genotyping ; Strongyloides ; Strongyloides fuelleborni ; Strongyloides stercoralis ; Strongyloidiasis Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) Method of publishing Limited access Institutional support UBO-W - RVO:68081766 UT WOS 000732747300008 EID SCOPUS 85119190687 DOI 10.1016/j.ijpara.2021.10.001 Annotation Strongyloidiasis represents a major medical and veterinary helminthic disease. Human infection is caused by Strongyloides stercoralis, Strongyloides fuelleborni fuelleborni and Strongyloides fuelleborni kellyi, with S. stercoralis accounting for the majority of cases. Strongyloides f. fuelleborni likely represents a zoonosis acquired from non-human primates (NHPs), while no animal reservoir for S. f. kellyi infection has been found. Whether S. stercoralis represents a zoonosis acquired from dogs and cats remains unanswered. Over the past two decades various tools have been applied to genotype Strongyloides spp. The most commonly sequenced markers have been the hyper-variable regions I and IV of the 18S rRNA gene and selected portions of the cytochrome c oxidase subunit I gene. These markers have been sequenced and compared in Strongyloides from multiple hosts and geographical regions. More recently, a machine learning algorithm multi-locus sequence typing approach has been applied using these markers, while others have applied whole genome sequencing. Genotyping of Strongyloides from dogs, cats, NHPs and humans has identified that S. stercoralis likely originated in dogs and adapted to human hosts. It has also been demonstrated that S. stercoralis is distinct from S. f. fuelleborni and S. f. kellyi. Two distinct genetic clades of S. stercoralis exist, one restricted to dogs and another infecting humans, NHPs, dogs and cats. Genotyping of S. f. fuelleborni has identified two separate clades, one associated with African isolates and another Indochinese peninsular clade. This review summarises the history and development of genotyping tools for Strongyloides spp. It describes the findings of major studies to date in the context of the epidemiology and evolutionary biology of these helminths, with a specific focus on human-infecting species. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0020751921002976?via%3Dihub
Number of the records: 1