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The B chromosome of Sorghum purpureosericeum reveals the first pieces of its sequence
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SYSNO ASEP 0545863 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The B chromosome of Sorghum purpureosericeum reveals the first pieces of its sequence Author(s) Karafiátová, Miroslava (UEB-Q) RID, ORCID
Bednářová, Martina (UEB-Q) ORCID
Said, Mahmoud (UEB-Q) ORCID
Čížková, Jana (UEB-Q) RID, ORCID
Holušová, Kateřina (UEB-Q) ORCID
Blavet, Nicolas (UEB-Q) RID, ORCID
Bartoš, Jan (UEB-Q) RID, ORCIDNumber of authors 7 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 72, č. 5 (2021), s. 1606-1616Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords B chromosomes ; cytogenetics ; flow cytometry ; pollen nuclei ; repeat analysis ; Sorghum purpureosericeum OECD category Biochemistry and molecular biology R&D Projects GJ18-12338Y GA ČR - Czech Science Foundation (CSF) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015047 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000637289800011 EID SCOPUS 85102129196 DOI https://doi.org/10.1093/jxb/eraa548 Annotation More than a century has passed since the B chromosomes were first discovered. Today we know much of their variability, morphology, and transmission to plant progeny. With the advent of modern technologies, B chromosome research has accelerated, and some of their persistent mysteries have since been uncovered. Building on this momentum, here we extend current knowledge of B chromosomes in Sorghum purpureosericeum to the sequence level. To do this, we estimated the B chromosome size at 421 Mb, sequenced DNA from flow-sorted haploid pollen nuclei of both B-positive (B+) and B-negative (B0) plants, and performed a repeat analysis on the Illumina raw sequence data. This analysis revealed nine putative B-specific clusters, which were then used to develop B chromosome-specific markers. Additionally, cluster SpuCL4 was identified and verified to be a centromeric repeat. We also uncovered two repetitive clusters (SpuCL168 and SpuCL115), which hybridized exclusively on the B chromosome under fluorescence in situ hybridization and can be considered as robust cytogenetic markers. Given that B chromosomes in Sorghum are rather unstable across all tissues, our findings could facilitate expedient identification of B+ plants and enable a wide range of studies to track this chromosome type in situ. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1093/jxb/eraa548
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